Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2636824..2637326 Replicon chromosome
Accession NZ_CP035767
Organism Escherichia coli O145 strain RM12522-C8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EW647_RS13810 Protein ID WP_000767829.1
Coordinates 2636824..2637078 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EW647_RS13815 Protein ID WP_001259255.1
Coordinates 2637075..2637326 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW647_RS13780 2631839..2632066 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EW647_RS13785 2632080..2633138 - 1059 WP_000492332.1 transport protein YeeE -
EW647_RS13790 2633317..2634675 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EW647_RS13795 2634665..2634727 - 63 WP_010723108.1 membrane protein YoeI -
EW647_RS13800 2634942..2635871 - 930 WP_000803351.1 LysR family transcriptional regulator -
EW647_RS13805 2635917..2636741 - 825 WP_000754755.1 SDR family oxidoreductase -
EW647_RS13810 2636824..2637078 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EW647_RS13815 2637075..2637326 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EW647_RS13820 2637610..2637660 + 51 WP_001364200.1 his operon leader peptide -
EW647_RS13825 2637806..2638705 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EW647_RS13830 2638711..2640015 + 1305 WP_001400693.1 histidinol dehydrogenase -
EW647_RS13835 2640012..2641082 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
EW647_RS13840 2641082..2642149 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T118418 WP_000767829.1 NZ_CP035767:c2637078-2636824 [Escherichia coli O145]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T118418 NZ_CP035767:c2637078-2636824 [Escherichia coli O145]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT118418 WP_001259255.1 NZ_CP035767:c2637326-2637075 [Escherichia coli O145]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT118418 NZ_CP035767:c2637326-2637075 [Escherichia coli O145]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References