Detailed information of TA system TA118397

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5592..5861
• Replicon: plasmid pE110019_66
• Accession: NZ_CP035752
• Organism: Escherichia coli E110019

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECE110019_RS25855
• Protein ID: WP_001312861.1
• Coordinates: 5703..5861 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	5592..5657 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE110019_RS25830	1359..1898	+	540	WP_000290791.1	single-stranded DNA-binding protein	-
ECE110019_RS25835	1961..2194	+	234	WP_000005990.1	DUF905 family protein	-
ECE110019_RS25840	2260..4218	+	1959	WP_000117175.1	ParB/RepB/Spo0J family partition protein	-
ECE110019_RS25845	4273..4707	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
ECE110019_RS25850	4704..5423	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
-	5435..5659	+	225	NuclAT_0	-	-
-	5435..5659	+	225	NuclAT_0	-	-
-	5435..5659	+	225	NuclAT_0	-	-
-	5435..5659	+	225	NuclAT_0	-	-
-	5592..5657	+	66	-	-	Antitoxin
ECE110019_RS25855	5703..5861	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ECE110019_RS25860	6215..6427	-	213	WP_162817365.1	hypothetical protein	-
ECE110019_RS25870	6783..7070	+	288	WP_000107535.1	hypothetical protein	-
ECE110019_RS25875	7188..8009	+	822	WP_001234469.1	DUF945 domain-containing protein	-
ECE110019_RS25880	8306..8896	-	591	WP_165899088.1	transglycosylase SLT domain-containing protein	-
ECE110019_RS25885	9229..9612	+	384	WP_001151524.1	relaxosome protein TraM	-
ECE110019_RS25890	9799..10488	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkJ / mrkI	1..66171	66171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T118397 WP_001312861.1 NZ_CP035752:5703-5861 [Escherichia coli E110019]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T118397 NZ_CP035752:5703-5861 [Escherichia coli E110019]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT118397 NZ_CP035752:5592-5657 [Escherichia coli E110019]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin