Detailed information of TA system TA118394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4896645..4897247
• Replicon: chromosome
• Accession: NZ_CP035751
• Organism: Escherichia coli E110019

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECE110019_RS24195
• Protein ID: WP_000897305.1
• Coordinates: 4896645..4896956 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECE110019_RS24200
• Protein ID: WP_000356397.1
• Coordinates: 4896957..4897247 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE110019_RS24170	4892559..4893158	+	600	WP_001295269.1	glucose-1-phosphatase	-
ECE110019_RS24175	4893152..4894024	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECE110019_RS24180	4894021..4894458	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECE110019_RS24185	4894503..4895444	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECE110019_RS24190	4895508..4896416	-	909	WP_001299484.1	alpha/beta hydrolase	-
ECE110019_RS24195	4896645..4896956	+	312	WP_000897305.1	hypothetical protein	Toxin
ECE110019_RS24200	4896957..4897247	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECE110019_RS24205	4897833..4898051	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ECE110019_RS24210	4898270..4898512	+	243	WP_001086388.1	hypothetical protein	-
ECE110019_RS24215	4898742..4899722	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
ECE110019_RS24220	4900121..4901050	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
ECE110019_RS24225	4901047..4901682	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4898742..4899722	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T118394 WP_000897305.1 NZ_CP035751:4896645-4896956 [Escherichia coli E110019]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118394 NZ_CP035751:4896645-4896956 [Escherichia coli E110019]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAAGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118394 WP_000356397.1 NZ_CP035751:4896957-4897247 [Escherichia coli E110019]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118394 NZ_CP035751:4896957-4897247 [Escherichia coli E110019]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7