Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2750764..2751266 Replicon chromosome
Accession NZ_CP035751
Organism Escherichia coli E110019

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECE110019_RS13715 Protein ID WP_000767829.1
Coordinates 2750764..2751018 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECE110019_RS13720 Protein ID WP_001259255.1
Coordinates 2751015..2751266 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE110019_RS13685 2745780..2746007 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECE110019_RS13690 2746021..2747079 - 1059 WP_000492320.1 transport protein YeeE -
ECE110019_RS13695 2747258..2748616 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECE110019_RS13700 2748606..2748668 - 63 WP_010723108.1 membrane protein YoeI -
ECE110019_RS13705 2748882..2749811 - 930 WP_000803360.1 LysR family transcriptional regulator -
ECE110019_RS13710 2749857..2750681 - 825 WP_000754755.1 SDR family oxidoreductase -
ECE110019_RS13715 2750764..2751018 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECE110019_RS13720 2751015..2751266 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECE110019_RS13725 2751550..2751600 + 51 WP_001364200.1 his operon leader peptide -
ECE110019_RS13730 2751746..2752645 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECE110019_RS13735 2752651..2753955 + 1305 WP_001299913.1 histidinol dehydrogenase -
ECE110019_RS13740 2753952..2755031 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
ECE110019_RS13745 2755028..2756095 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T118389 WP_000767829.1 NZ_CP035751:c2751018-2750764 [Escherichia coli E110019]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T118389 NZ_CP035751:c2751018-2750764 [Escherichia coli E110019]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT118389 WP_001259255.1 NZ_CP035751:c2751266-2751015 [Escherichia coli E110019]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT118389 NZ_CP035751:c2751266-2751015 [Escherichia coli E110019]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References