Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 780025..780566 Replicon chromosome
Accession NZ_CP035751
Organism Escherichia coli E110019

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag ECE110019_RS03660 Protein ID WP_000615976.1
Coordinates 780288..780566 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag ECE110019_RS03655 Protein ID WP_000729704.1
Coordinates 780025..780285 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE110019_RS03630 775808..776593 - 786 WP_000207559.1 putative lateral flagellar export/assembly protein LafU -
ECE110019_RS03635 776565..778277 + 1713 Protein_712 flagellar biosynthesis protein FlhA -
ECE110019_RS03640 778382..778660 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
ECE110019_RS03645 778653..779009 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
ECE110019_RS03650 779066..779839 - 774 WP_000543899.1 C40 family peptidase -
ECE110019_RS03655 780025..780285 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECE110019_RS03660 780288..780566 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECE110019_RS03665 780722..781462 + 741 WP_001225679.1 murein L,D-transpeptidase -
ECE110019_RS03670 781433..782200 - 768 WP_000333380.1 class II glutamine amidotransferase -
ECE110019_RS03675 782406..782984 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T118375 WP_000615976.1 NZ_CP035751:780288-780566 [Escherichia coli E110019]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T118375 NZ_CP035751:780288-780566 [Escherichia coli E110019]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCTGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT118375 WP_000729704.1 NZ_CP035751:780025-780285 [Escherichia coli E110019]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT118375 NZ_CP035751:780025-780285 [Escherichia coli E110019]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References