Detailed information of TA system TA118370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5213276..5213871
• Replicon: chromosome
• Accession: NZ_CP035739
• Organism: Pseudomonas aeruginosa strain 1334/14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: EWS90_RS25210
• Protein ID: WP_003117425.1
• Coordinates: 5213276..5213554 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EWS90_RS25215
• Protein ID: WP_003113527.1
• Coordinates: 5213566..5213871 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWS90_RS25190	5208702..5209940	+	1239	WP_003162396.1	C69 family dipeptidase	-
EWS90_RS25195	5210002..5210649	+	648	WP_003095021.1	carbonate dehydratase	-
EWS90_RS25200	5210719..5212947	-	2229	WP_003113525.1	TonB-dependent receptor	-
EWS90_RS25210	5213276..5213554	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EWS90_RS25215	5213566..5213871	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
EWS90_RS25225	5214278..5215378	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
EWS90_RS25230	5215419..5216003	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
EWS90_RS25235	5216045..5216659	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
EWS90_RS25240	5216776..5217717	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
EWS90_RS25250	5217884..5218732	-	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T118370 WP_003117425.1 NZ_CP035739:5213276-5213554 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T118370 NZ_CP035739:5213276-5213554 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT118370 WP_003113527.1 NZ_CP035739:5213566-5213871 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT118370 NZ_CP035739:5213566-5213871 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5