Detailed information of TA system TA118365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4761863..4762593
• Replicon: chromosome
• Accession: NZ_CP035738
• Organism: Enterobacter cloacae strain CZ-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W2Z921
• Locus tag: EWI30_RS23245
• Protein ID: WP_032662028.1
• Coordinates: 4762279..4762593 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EWI30_RS23240
• Protein ID: WP_165383613.1
• Coordinates: 4761863..4762282 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWI30_RS23215	4757358..4758863	-	1506	WP_129997012.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
EWI30_RS23220	4758860..4759453	-	594	WP_114979020.1	transcriptional regulator UhpA	-
EWI30_RS23225	4759531..4759818	-	288	WP_033144334.1	acetolactate synthase small subunit	-
EWI30_RS23230	4759822..4761510	-	1689	WP_129997013.1	acetolactate synthase large subunit	-
EWI30_RS23235	4761617..4761715	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
EWI30_RS23240	4761863..4762282	-	420	WP_165383613.1	helix-turn-helix domain-containing protein	Antitoxin
EWI30_RS23245	4762279..4762593	-	315	WP_032662028.1	type II toxin-antitoxin system HigB family toxin	Toxin
EWI30_RS23250	4763079..4763168	+	90	WP_020684667.1	type I toxin-antitoxin system toxin TisB	-
EWI30_RS23255	4763233..4763679	+	447	WP_096150935.1	GNAT family N-acetyltransferase	-
EWI30_RS23260	4763742..4764575	+	834	WP_129997014.1	DMT family transporter	-
EWI30_RS23265	4764751..4765935	+	1185	WP_129997015.1	multidrug efflux MFS transporter EmrD	-
EWI30_RS23270	4765838..4766926	-	1089	WP_129997016.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12375.48 Da
• Isoelectric Point: 10.2219

>T118365 WP_032662028.1 NZ_CP035738:c4762593-4762279 [Enterobacter cloacae]
MHLISMKAILDAVTQFPQHKEELLFLGRTIEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T118365 NZ_CP035738:c4762593-4762279 [Enterobacter cloacae]
ATGCATTTAATCTCTATGAAAGCTATTTTGGATGCGGTAACTCAGTTTCCGCAGCACAAAGAAGAACTTCTCTTTTTGGG
CCGCACCATCGAAAAGAGCCATTGTCCGACGCCTGCTGCGCTGAAAAAACTGTTTCCGACGCTGGATAACTTTAAGTATC
TGGATAAGCATTATGTTATTGATATTGCAAACAACAATCTCAGAGTAGTGGCTCTCATTTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15539.12 Da
• Isoelectric Point: 5.2231

>AT118365 WP_165383613.1 NZ_CP035738:c4762282-4761863 [Enterobacter cloacae]
MMIVADAMKATHALVTAVPLLGEHPSEKDYKDALELVEYLLMNEPDSPLLDIVCARISRYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT118365 NZ_CP035738:c4762282-4761863 [Enterobacter cloacae]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTACGGCAGTTCCTTTACTGGGTGAACATCCGAGCGA
GAAAGATTATAAAGATGCGCTTGAGCTGGTTGAGTATCTGCTCATGAACGAGCCCGATAGCCCCTTGTTGGATATTGTGT
GCGCCAGGATTAGCCGTTACGAAGCCAATCGGCCTGAGATAGTCGCATTACGCAAGGAGATGGAGTCTGTCCCTGTCGGT
ATTGCGGTTTTAAGAACCTTAATGGATCAATACAACCTGACGATATCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCACGGGTTCTGAATGGTCAAAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CAGCTCTGTTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7W2Z921