118360

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3626016..3626743 Replicon chromosome
Accession NZ_CP035738
Organism Enterobacter cloacae strain CZ-1

Toxin (Protein)


Gene name higB Uniprot ID A0A8B2UR49
Locus tag EWI30_RS17795 Protein ID WP_045631527.1
Coordinates 3626016..3626327 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EWI30_RS17800 Protein ID WP_114978029.1
Coordinates 3626324..3626743 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWI30_RS17770 3621854..3622108 + 255 WP_114978034.1 hypothetical protein -
EWI30_RS17775 3622165..3622770 - 606 WP_114978033.1 glutathione transferase GstA -
EWI30_RS17780 3622891..3623775 + 885 WP_129996507.1 LysR family transcriptional regulator -
EWI30_RS17785 3624188..3624643 + 456 WP_129996508.1 hypothetical protein -
EWI30_RS17790 3624885..3625667 + 783 WP_129997127.1 DUF2971 domain-containing protein -
EWI30_RS17795 3626016..3626327 + 312 WP_045631527.1 type II toxin-antitoxin system HigB family toxin Toxin
EWI30_RS17800 3626324..3626743 + 420 WP_114978029.1 helix-turn-helix domain-containing protein Antitoxin
EWI30_RS17805 3627081..3627485 - 405 WP_129996509.1 SMI1/KNR4 family protein -
EWI30_RS17810 3627551..3627988 - 438 WP_129996510.1 hypothetical protein -
EWI30_RS17815 3628176..3628560 - 385 Protein_3421 immunity 50 family protein -
EWI30_RS17820 3628749..3629195 - 447 WP_114978026.1 type IV secretion protein Rhs -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Antitoxin HigA

(5-137)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12402.28 Da        Isoelectric Point: 10.0936

>T118360 WP_045631527.1 NZ_CP035738:3626016-3626327 [Enterobacter cloacae]
MHVVSRAPFDAATRQFPNQALALDDLYRVLKKEHYPSPDDMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHAEYDKLTDLYRRTKE

Download         Length: 312 bp

>T118360 NZ_CP035738:3626016-3626327 [Enterobacter cloacae]
ATGCACGTAGTCTCAAGAGCACCTTTCGACGCAGCCACCAGACAATTTCCTAATCAAGCACTCGCTCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTATCCATCACCAGACGACATGCGAAAGCGCTTTCCCAGCCTGGATCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTAGGTGGCGGGCATTTAAGAGTGATGTTTTTTGCTGATTTTGAGCGGGGAAAGATC
TTCATCAAACACATCAGCACTCACGCAGAGTATGACAAGCTCACGGACCTCTACCGGAGAACGAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15529.57 Da        Isoelectric Point: 4.6268

>AT118360 WP_114978029.1 NZ_CP035738:3626324-3626743 [Enterobacter cloacae]
MNYAAAIKAANDLTSELPFLGSRPSRQDYEDALGLVEYLIEYDPDSPLVEMLTTKIDKYENESPEFAEFNARVASIPSGV
ALLRTLMDQYHLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGVPVSAFVGN

Download         Length: 420 bp

>AT118360 NZ_CP035738:3626324-3626743 [Enterobacter cloacae]
ATGAACTATGCGGCAGCCATCAAAGCGGCAAACGATCTGACAAGCGAGCTTCCTTTCCTGGGTTCAAGACCGTCGCGTCA
AGATTACGAGGATGCTCTGGGATTGGTGGAATATCTGATTGAGTATGACCCCGATAGCCCGCTGGTAGAGATGCTCACTA
CGAAGATCGATAAATACGAAAACGAGTCTCCGGAGTTTGCAGAATTTAACGCGCGTGTAGCCAGTATTCCGTCAGGCGTT
GCGTTATTGCGAACGCTGATGGATCAGTACCATCTCACACAGAGCGATTTTGAGAATGAGATAGGCAAGAAATCTTTGGT
GAGTCGAATCCTCAACGGACAGCGCACGTTAACGCTGGATCATATGCGCGCGCTGGCGAAGCGTTTTGGCGTGCCGGTAA
GTGCATTTGTGGGGAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2UR49

Antitoxin

Source ID Structure

References