Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1090149..1090806 Replicon chromosome
Accession NZ_CP035738
Organism Enterobacter cloacae strain CZ-1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EWI30_RS05290 Protein ID WP_129995132.1
Coordinates 1090507..1090806 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EWI30_RS05285 Protein ID WP_047650330.1
Coordinates 1090149..1090496 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWI30_RS05255 1085762..1086649 + 888 WP_129995126.1 LysR family transcriptional regulator -
EWI30_RS05260 1086670..1087851 + 1182 WP_129995127.1 PLP-dependent aminotransferase family protein -
EWI30_RS05265 1087937..1088455 + 519 WP_129995128.1 hypothetical protein -
EWI30_RS05270 1088458..1088877 - 420 WP_129995129.1 nuclear transport factor 2 family protein -
EWI30_RS05275 1088975..1089706 + 732 WP_129995130.1 helix-turn-helix transcriptional regulator -
EWI30_RS05280 1089678..1090103 - 426 WP_129995131.1 nucleoside triphosphatase NudI -
EWI30_RS05285 1090149..1090496 - 348 WP_047650330.1 HigA family addiction module antidote protein Antitoxin
EWI30_RS05290 1090507..1090806 - 300 WP_129995132.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EWI30_RS05295 1091060..1092229 - 1170 WP_129997064.1 HlyD family efflux transporter periplasmic adaptor subunit -
EWI30_RS05300 1092240..1094435 - 2196 WP_129995133.1 type I secretion system permease/ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11651.25 Da        Isoelectric Point: 9.9226

>T118347 WP_129995132.1 NZ_CP035738:c1090806-1090507 [Enterobacter cloacae]
MINHFRDQWLEDFFLYGRSSKVIPSNLETALARKLDIINAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFLW
TEGKADDLYLSPHRYTQHK

Download         Length: 300 bp

>T118347 NZ_CP035738:c1090806-1090507 [Enterobacter cloacae]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGTAGATCGAGCAAGGTTATTCCTTCAAATCT
GGAGACAGCGCTTGCGAGAAAGCTCGACATCATCAACGCAGCTACCTCGCACCGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGATATTCCTCAATCCGGGTAAACAGGCAATACAGGCTTGTATTTCTCTGG
ACAGAGGGTAAAGCAGACGATCTCTACCTCTCTCCACACAGGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13070.01 Da        Isoelectric Point: 7.0139

>AT118347 WP_047650330.1 NZ_CP035738:c1090496-1090149 [Enterobacter cloacae]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGQPDVA

Download         Length: 348 bp

>AT118347 NZ_CP035738:c1090496-1090149 [Enterobacter cloacae]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTACGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTTAATGTACACCGCAATACCATCAGCGCGCTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATAAAACTGGCAAAAGCCTTTGATACCACTATTGAATTTTGGCTGAACCTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCTAACTCCCGGACGCAGGAGGAGTTAAGCCGTATAAAGACCGTTGCGGAAGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References