Detailed information of TA system TA118329

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62710..63136
• Replicon: plasmid pU15A_B
• Accession: NZ_CP035722
• Organism: Escherichia coli strain U15A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: U15A_RS25710
• Protein ID: WP_001312861.1
• Coordinates: 62710..62868 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	62912..63136 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U15A_RS25660	57724..57951	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
U15A_RS25665	58088..58759	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
U15A_RS25670	58953..59336	-	384	WP_001354030.1	relaxosome protein TraM	-
U15A_RS25680	59659..60261	+	603	WP_023908348.1	transglycosylase SLT domain-containing protein	-
U15A_RS25690	60556..61377	-	822	WP_001234469.1	DUF945 domain-containing protein	-
U15A_RS25695	61496..61783	-	288	WP_000107526.1	hypothetical protein	-
U15A_RS25710	62710..62868	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	62912..63136	-	225	NuclAT_0	-	Antitoxin
-	62912..63136	-	225	NuclAT_0	-	Antitoxin
-	62912..63136	-	225	NuclAT_0	-	Antitoxin
-	62912..63136	-	225	NuclAT_0	-	Antitoxin
U15A_RS26055	62948..63136	+	189	WP_001336239.1	hypothetical protein	-
U15A_RS25715	63148..63867	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
U15A_RS25720	63864..64298	-	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
U15A_RS25725	64353..66311	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
U15A_RS25730	66377..66610	-	234	WP_000005990.1	DUF905 family protein	-
U15A_RS25735	66673..67212	-	540	WP_000290841.1	single-stranded DNA-binding protein	-
U15A_RS25760	67855..68091	-	237	WP_032153960.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..69541	69541

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T118329 WP_001312861.1 NZ_CP035722:c62868-62710 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T118329 NZ_CP035722:c62868-62710 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT118329 NZ_CP035722:c63136-62912 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGTCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin