Detailed information of TA system TA118325

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26418..26682
• Replicon: plasmid pU15A_A
• Accession: NZ_CP035721
• Organism: Escherichia coli strain U15A

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: U15A_RS24605
• Protein ID: WP_001331364.1
• Coordinates: 26418..26570 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	26625..26682 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U15A_RS24585	21695..23863	+	2169	WP_125075213.1	DotA/TraY family protein	-
U15A_RS24590	23937..24587	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
U15A_RS24595	24659..24868	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
U15A_RS26035	25260..25436	+	177	WP_001054897.1	hypothetical protein	-
U15A_RS24600	26095..26346	+	252	WP_001291964.1	hypothetical protein	-
U15A_RS24605	26418..26570	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	26625..26682	+	58	NuclAT_0	-	Antitoxin
-	26625..26682	+	58	NuclAT_0	-	Antitoxin
-	26625..26682	+	58	NuclAT_0	-	Antitoxin
-	26625..26682	+	58	NuclAT_0	-	Antitoxin
U15A_RS24610	26862..28070	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
U15A_RS24615	28089..29159	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
U15A_RS24620	29152..31443	+	2292	WP_001289276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / aac(3)-IId	-	1..109023	109023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T118325 WP_001331364.1 NZ_CP035721:c26570-26418 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T118325 NZ_CP035721:c26570-26418 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT118325 NZ_CP035721:26625-26682 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin