Detailed information of TA system TA118315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2249618..2250120
• Replicon: chromosome
• Accession: NZ_CP035720
• Organism: Escherichia coli strain U15A

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: U15A_RS11580
• Protein ID: WP_000767819.1
• Coordinates: 2249618..2249872 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: U15A_RS11585
• Protein ID: WP_001259253.1
• Coordinates: 2249869..2250120 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U15A_RS11550	2245436..2245894	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
U15A_RS11560	2246111..2247469	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
U15A_RS11565	2247459..2247521	-	63	WP_010723108.1	membrane protein YoeI	-
U15A_RS11570	2247736..2248665	-	930	WP_000803366.1	LysR family transcriptional regulator	-
U15A_RS11575	2248711..2249535	-	825	WP_000754737.1	SDR family oxidoreductase	-
U15A_RS11580	2249618..2249872	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
U15A_RS11585	2249869..2250120	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
U15A_RS11595	2250403..2250453	+	51	WP_001364200.1	his operon leader peptide	-
U15A_RS11600	2250599..2251498	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
U15A_RS11605	2251504..2252808	+	1305	WP_001296211.1	histidinol dehydrogenase	-
U15A_RS11610	2252805..2253875	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
U15A_RS11615	2253875..2254942	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2245436..2245894	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T118315 WP_000767819.1 NZ_CP035720:c2249872-2249618 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T118315 NZ_CP035720:c2249872-2249618 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT118315 WP_001259253.1 NZ_CP035720:c2250120-2249869 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT118315 NZ_CP035720:c2250120-2249869 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3