Detailed information of TA system TA118283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 460210..460843
• Replicon: chromosome
• Accession: NZ_CP035698
• Organism: Vibrio cincinnatiensis strain 2409-02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: ET948_RS15125
• Protein ID: WP_000843587.1
• Coordinates: 460511..460843 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ET948_RS15120
• Protein ID: WP_000071008.1
• Coordinates: 460210..460524 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ET948_RS15095	455682..455903	-	222	Protein_414	transposase	-
ET948_RS15100	456094..456528	-	435	WP_154116308.1	hypothetical protein	-
ET948_RS15105	456660..457124	-	465	WP_154116310.1	hypothetical protein	-
ET948_RS15110	457196..458320	-	1125	WP_154116312.1	ISAs1 family transposase	-
ET948_RS15115	459127..459423	-	297	WP_053339486.1	PQQ-binding-like beta-propeller repeat protein	-
ET948_RS15120	460210..460524	-	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
ET948_RS15125	460511..460843	-	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ET948_RS15130	460911..461054	-	144	WP_195707336.1	DUF3265 domain-containing protein	-
ET948_RS15135	461024..461425	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	-
ET948_RS15140	461425..461595	-	171	WP_001080654.1	hypothetical protein	-
ET948_RS15145	461775..463082	-	1308	WP_154116314.1	DUF3644 domain-containing protein	-
ET948_RS15150	463232..463591	-	360	WP_154116316.1	hypothetical protein	-
ET948_RS15155	463638..463754	-	117	Protein_426	DUF3265 domain-containing protein	-
ET948_RS15160	463751..464146	-	396	WP_154116906.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	384751..459423	74672
	-	flank	IS/Tn	-	-	465598..466554	956
	-	inside	Integron	-	-	436106..502859	66753

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T118283 WP_000843587.1 NZ_CP035698:c460843-460511 [Vibrio cincinnatiensis]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T118283 NZ_CP035698:c460843-460511 [Vibrio cincinnatiensis]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGACGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT118283 WP_000071008.1 NZ_CP035698:c460524-460210 [Vibrio cincinnatiensis]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT118283 NZ_CP035698:c460524-460210 [Vibrio cincinnatiensis]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCACGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA