Detailed information of TA system TA118208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3749156..3749783
• Replicon: chromosome
• Accession: NZ_CP035668
• Organism: Edwardsiella piscicida strain MS-18-199

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A411H7J1
• Locus tag: EVK84_RS17285
• Protein ID: WP_015871332.1
• Coordinates: 3749156..3749485 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EVK84_RS17290
• Protein ID: WP_005286394.1
• Coordinates: 3749475..3749783 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EVK84_RS17255	3744531..3745403	-	873	WP_129986145.1	antA/AntB antirepressor family protein	-
EVK84_RS17260	3745494..3746045	-	552	WP_129986147.1	glycoside hydrolase family 108 protein	-
EVK84_RS17265	3746048..3746395	-	348	WP_129986149.1	phage holin, lambda family	-
EVK84_RS17270	3746565..3747383	-	819	WP_129986151.1	hypothetical protein	-
EVK84_RS17275	3747476..3747748	-	273	WP_129986153.1	hypothetical protein	-
EVK84_RS18950	3747952..3748401	-	450	Protein_3316	hypothetical protein	-
EVK84_RS18955	3748390..3748710	-	321	Protein_3317	antA/AntB antirepressor family protein	-
EVK84_RS17285	3749156..3749485	+	330	WP_015871332.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EVK84_RS17290	3749475..3749783	+	309	WP_005286394.1	helix-turn-helix transcriptional regulator	Antitoxin
EVK84_RS17295	3749878..3750390	+	513	WP_012848469.1	DUF2442 domain-containing protein	-
EVK84_RS17300	3750473..3750841	-	369	WP_196796969.1	Txe/YoeB family addiction module toxin	-
EVK84_RS17305	3750729..3750974	-	246	WP_012848468.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EVK84_RS17310	3751264..3751566	-	303	WP_012848467.1	DUF1364 domain-containing protein	-
EVK84_RS17315	3751569..3752213	-	645	WP_073382273.1	DUF1367 family protein	-
EVK84_RS17320	3752323..3752670	-	348	WP_129986157.1	hypothetical protein	-
EVK84_RS17325	3752698..3754080	-	1383	WP_129986159.1	replicative DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3712864..3820549	107685

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12771.78 Da
• Isoelectric Point: 9.6247

>T118208 WP_015871332.1 NZ_CP035668:3749156-3749485 [Edwardsiella piscicida]
MNYTIEYYSEEVRLEVDQLPMGMRVRYQHLVERMEIYGSNLGEPHTSPFGDGLFELRIKGSDGIARVFYCTLTGKRIVML
HSFIKKTQKTPSAERKKAETRMKEVKHGW

Nucleotide

• Length: 330 bp

>T118208 NZ_CP035668:3749156-3749485 [Edwardsiella piscicida]
ATGAACTACACTATTGAGTACTACAGCGAAGAGGTTCGGCTTGAGGTCGATCAGTTGCCAATGGGTATGCGGGTTCGATA
CCAGCATCTCGTTGAACGCATGGAAATCTACGGTAGTAATCTCGGAGAACCACACACAAGCCCTTTTGGCGATGGGCTTT
TTGAGCTTCGAATCAAAGGCAGTGATGGCATCGCCCGCGTGTTTTACTGCACTCTTACCGGAAAACGTATCGTCATGCTG
CACAGCTTCATCAAGAAAACCCAGAAGACGCCAAGCGCTGAACGTAAGAAGGCTGAAACCAGAATGAAGGAGGTAAAACA
TGGCTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11233.90 Da
• Isoelectric Point: 8.5371

>AT118208 WP_005286394.1 NZ_CP035668:3749475-3749783 [Edwardsiella piscicida]
MAGKRTPPTMTHDEMAAKWMEDPAFKAEYDAIEEEYALLDEMLAARKNAGLTQAQVAERMGTKPTAITRMESNLASGQSG
PSFATLKKFAHATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT118208 NZ_CP035668:3749475-3749783 [Edwardsiella piscicida]
ATGGCTGGTAAACGCACCCCCCCCACGATGACGCATGATGAAATGGCTGCAAAATGGATGGAAGACCCGGCATTTAAAGC
AGAATACGATGCCATCGAAGAGGAATACGCCCTACTCGATGAAATGCTCGCCGCCCGCAAAAACGCGGGGCTGACCCAGG
CGCAGGTCGCCGAACGTATGGGAACCAAGCCTACAGCGATCACTCGCATGGAAAGTAATTTAGCCTCCGGCCAAAGCGGG
CCATCATTCGCCACGCTGAAAAAATTTGCACACGCGACCGGCAAGAAACTGCAGATCCGGTTTGTCTGA

Structures

Toxin

• AlphaFold DB: A0A411H7J1