Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 242064..242599 Replicon plasmid unnamed1
Accession NZ_CP035655
Organism Enterococcus faecium strain UAMSEF_08

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag EWM54_RS16240 Protein ID WP_002314366.1
Coordinates 242064..242336 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag EWM54_RS16245 Protein ID WP_002297422.1
Coordinates 242336..242599 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWM54_RS16200 237202..237846 + 645 Protein_269 transposase -
EWM54_RS16205 237922..238602 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
EWM54_RS16210 238661..238849 - 189 Protein_271 hypothetical protein -
EWM54_RS16215 238850..239512 - 663 Protein_272 AAA family ATPase -
EWM54_RS16225 240902..241097 - 196 Protein_274 ParA family protein -
EWM54_RS16230 241382..241668 - 287 Protein_275 type II toxin-antitoxin system RelE/ParE family toxin -
EWM54_RS16235 241658..241987 - 330 WP_002292678.1 AbrB family transcriptional regulator -
EWM54_RS16240 242064..242336 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
EWM54_RS16245 242336..242599 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EWM54_RS16250 242833..243741 + 909 Protein_279 IS982 family transposase -
EWM54_RS16255 243744..244919 - 1176 WP_002336205.1 MFS transporter -
EWM54_RS16260 244922..247171 - 2250 WP_106017163.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..256961 256961
- inside IScluster/Tn - - 229109..255589 26480


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T118188 WP_002314366.1 NZ_CP035655:c242336-242064 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T118188 NZ_CP035655:c242336-242064 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT118188 WP_002297422.1 NZ_CP035655:c242599-242336 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT118188 NZ_CP035655:c242599-242336 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References