Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 242130..242665 Replicon plasmid unnamed1
Accession NZ_CP035649
Organism Enterococcus faecium strain UAMSEF_01

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag EWM53_RS16220 Protein ID WP_002314366.1
Coordinates 242130..242402 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag EWM53_RS16225 Protein ID WP_002297422.1
Coordinates 242402..242665 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWM53_RS16180 237269..237912 + 644 Protein_268 transposase -
EWM53_RS16185 237988..238668 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
EWM53_RS16190 238727..238915 - 189 Protein_270 hypothetical protein -
EWM53_RS16195 238916..239578 - 663 Protein_271 AAA family ATPase -
EWM53_RS16205 240968..241162 - 195 Protein_273 ParA family protein -
EWM53_RS16210 241448..241734 - 287 Protein_274 type II toxin-antitoxin system RelE/ParE family toxin -
EWM53_RS16215 241724..242053 - 330 WP_002292678.1 AbrB family transcriptional regulator -
EWM53_RS16220 242130..242402 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
EWM53_RS16225 242402..242665 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EWM53_RS16230 242899..243808 + 910 Protein_278 IS982 family transposase -
EWM53_RS16235 243811..244986 - 1176 WP_002336205.1 MFS transporter -
EWM53_RS16240 244989..247238 - 2250 WP_106017163.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..257028 257028
- inside IScluster/Tn - - 229177..255656 26479


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T118183 WP_002314366.1 NZ_CP035649:c242402-242130 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T118183 NZ_CP035649:c242402-242130 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT118183 WP_002297422.1 NZ_CP035649:c242665-242402 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT118183 NZ_CP035649:c242665-242402 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References