Detailed information of TA system TA118169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3191226..3191883
• Replicon: chromosome
• Accession: NZ_CP035633
• Organism: Enterobacter cloacae strain EN3600

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A808IF03
• Locus tag: EVV94_RS16130
• Protein ID: WP_033146338.1
• Coordinates: 3191226..3191525 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EVV94_RS16135
• Protein ID: WP_094935697.1
• Coordinates: 3191536..3191883 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EVV94_RS16115	3187276..3187599	+	324	Protein_3123	type I secretion protein TolC	-
EVV94_RS16120	3187596..3189794	+	2199	WP_094935698.1	type I secretion system permease/ATPase	-
EVV94_RS16125	3189805..3190974	+	1170	WP_086374946.1	HlyD family efflux transporter periplasmic adaptor subunit	-
EVV94_RS16130	3191226..3191525	+	300	WP_033146338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EVV94_RS16135	3191536..3191883	+	348	WP_094935697.1	HigA family addiction module antidote protein	Antitoxin
EVV94_RS16140	3191928..3192353	+	426	WP_033146340.1	nucleoside triphosphatase NudI	-
EVV94_RS16145	3192325..3193056	-	732	WP_094935696.1	helix-turn-helix transcriptional regulator	-
EVV94_RS16150	3193154..3193573	+	420	WP_039263434.1	nuclear transport factor 2 family protein	-
EVV94_RS16155	3193576..3194094	-	519	WP_094935695.1	hypothetical protein	-
EVV94_RS16160	3194179..3195360	-	1182	WP_094935694.1	PLP-dependent aminotransferase family protein	-
EVV94_RS16165	3195381..3196268	-	888	WP_094935693.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3162861..3191883	29022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11691.32 Da
• Isoelectric Point: 10.0346

>T118169 WP_033146338.1 NZ_CP035633:3191226-3191525 [Enterobacter cloacae]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T118169 NZ_CP035633:3191226-3191525 [Enterobacter cloacae]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTAAAGGGATATTCCTCAATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13084.05 Da
• Isoelectric Point: 7.0134

>AT118169 WP_094935697.1 NZ_CP035633:3191536-3191883 [Enterobacter cloacae]
MTLQQALRKPTTPGDVMQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT118169 NZ_CP035633:3191536-3191883 [Enterobacter cloacae]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGATGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTGA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A808IF03