Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2470775..2471435 Replicon chromosome
Accession NZ_CP035633
Organism Enterobacter cloacae strain EN3600

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EVV94_RS12320 Protein ID WP_094933998.1
Coordinates 2471082..2471435 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EVV94_RS12315 Protein ID WP_094933997.1
Coordinates 2470775..2471077 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVV94_RS12295 2466630..2468819 + 2190 WP_048977175.1 TonB-dependent siderophore receptor -
EVV94_RS12305 2469249..2469638 - 390 WP_094933995.1 RidA family protein -
EVV94_RS12310 2469780..2470700 + 921 WP_094933996.1 LysR family transcriptional regulator -
EVV94_RS12315 2470775..2471077 - 303 WP_094933997.1 XRE family transcriptional regulator Antitoxin
EVV94_RS12320 2471082..2471435 - 354 WP_094933998.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EVV94_RS12325 2471612..2472505 - 894 WP_094933999.1 LysR family transcriptional regulator -
EVV94_RS12330 2472676..2473284 + 609 WP_094934000.1 glutathione S-transferase family protein -
EVV94_RS12335 2473328..2474278 - 951 WP_023336219.1 HTH-type transcriptional regulator Cbl -
EVV94_RS12340 2474375..2475292 - 918 WP_008499475.1 nitrogen assimilation transcriptional regulator NAC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13543.42 Da        Isoelectric Point: 9.0310

>T118168 WP_094933998.1 NZ_CP035633:c2471435-2471082 [Enterobacter cloacae]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
ILCAGNKVGNERRFYDEMLLVADREFTHWLNILKERE

Download         Length: 354 bp

>T118168 NZ_CP035633:c2471435-2471082 [Enterobacter cloacae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACATCGCTTGACGATACCGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCCCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCATTACGGGCGTTTTTCGCCTTTGACCCAAACCGTACTGGCATC
ATACTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCGGGAATTCAC
TCACTGGCTGAATATATTAAAAGAGAGGGAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11050.83 Da        Isoelectric Point: 5.7316

>AT118168 WP_094933997.1 NZ_CP035633:c2471077-2470775 [Enterobacter cloacae]
MGRTLEQLIADEKPEVVASAQALATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT118168 NZ_CP035633:c2471077-2470775 [Enterobacter cloacae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGCTAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCTGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGATGGCTCGCACTATGAGTTTGTGCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References