Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4094215..4094861 Replicon chromosome
Accession NZ_CP035531
Organism Klebsiella pneumoniae strain NFYY0065

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag EI683_RS20480 Protein ID WP_016529833.1
Coordinates 4094514..4094861 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag EI683_RS20475 Protein ID WP_002920557.1
Coordinates 4094215..4094514 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EI683_RS20455 4090421..4091179 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
EI683_RS20460 4091229..4092059 - 831 WP_024622927.1 rhomboid family intramembrane serine protease GlpG -
EI683_RS20465 4092110..4092439 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
EI683_RS20470 4092644..4094152 + 1509 WP_023301456.1 glycerol-3-phosphate dehydrogenase -
EI683_RS20475 4094215..4094514 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
EI683_RS20480 4094514..4094861 - 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EI683_RS20485 4095037..4097484 - 2448 WP_002920561.1 glycogen phosphorylase -
EI683_RS20490 4097502..4098935 - 1434 WP_023342584.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T118078 WP_016529833.1 NZ_CP035531:c4094861-4094514 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T118078 NZ_CP035531:c4094861-4094514 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT118078 WP_002920557.1 NZ_CP035531:c4094514-4094215 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT118078 NZ_CP035531:c4094514-4094215 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References