Detailed information of TA system TA118046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4345911..4346513
• Replicon: chromosome
• Accession: NZ_CP035516
• Organism: Escherichia coli strain U14A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: U14A_RS21625
• Protein ID: WP_000897302.1
• Coordinates: 4345911..4346222 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: U14A_RS21630
• Protein ID: WP_000356397.1
• Coordinates: 4346223..4346513 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U14A_RS21600	4341825..4342424	+	600	WP_001296610.1	glucose-1-phosphatase	-
U14A_RS21605	4342418..4343290	+	873	WP_000920762.1	virulence factor BrkB family protein	-
U14A_RS21610	4343287..4343724	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
U14A_RS21615	4343769..4344710	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
U14A_RS21620	4344774..4345682	-	909	WP_001385591.1	alpha/beta hydrolase	-
U14A_RS21625	4345911..4346222	+	312	WP_000897302.1	hypothetical protein	Toxin
U14A_RS21630	4346223..4346513	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
U14A_RS21640	4346872..4347150	+	279	WP_001296612.1	hypothetical protein	-
U14A_RS21650	4347547..4347765	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
U14A_RS21655	4347950..4348399	-	450	WP_032140890.1	hypothetical protein	-
U14A_RS21660	4348715..4349563	-	849	WP_001038650.1	hypothetical protein	-
U14A_RS21665	4349853..4350095	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
U14A_RS21670	4350277..4351206	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T118046 WP_000897302.1 NZ_CP035516:4345911-4346222 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118046 NZ_CP035516:4345911-4346222 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118046 WP_000356397.1 NZ_CP035516:4346223-4346513 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118046 NZ_CP035516:4346223-4346513 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416