Detailed information of TA system TA118010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5135685..5136287
• Replicon: chromosome
• Accession: NZ_CP035498
• Organism: Escherichia coli strain 04-00955

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: EQO00_RS25410
• Protein ID: WP_000897302.1
• Coordinates: 5135976..5136287 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EQO00_RS25405
• Protein ID: WP_000356397.1
• Coordinates: 5135685..5135975 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQO00_RS25370	5131758..5132660	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EQO00_RS25375	5132657..5133292	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EQO00_RS25380	5133289..5134218	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
EQO00_RS25385	5134434..5134652	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
EQO00_RS25395	5135048..5135326	-	279	WP_001296612.1	hypothetical protein	-
EQO00_RS25405	5135685..5135975	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EQO00_RS25410	5135976..5136287	-	312	WP_000897302.1	hypothetical protein	Toxin
EQO00_RS25415	5136516..5137424	+	909	WP_001331553.1	alpha/beta hydrolase	-
EQO00_RS25420	5137488..5138429	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EQO00_RS25425	5138474..5138911	-	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EQO00_RS25430	5138908..5139780	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EQO00_RS25435	5139774..5140373	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T118010 WP_000897302.1 NZ_CP035498:c5136287-5135976 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T118010 NZ_CP035498:c5136287-5135976 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT118010 WP_000356397.1 NZ_CP035498:c5135975-5135685 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT118010 NZ_CP035498:c5135975-5135685 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416