Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4091047..4091741 Replicon chromosome
Accession NZ_CP035498
Organism Escherichia coli strain 04-00955

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag EQO00_RS20375 Protein ID WP_001263501.1
Coordinates 4091047..4091445 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag EQO00_RS20380 Protein ID WP_000554755.1
Coordinates 4091448..4091741 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQO00_RS20350 4086412..4086870 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
EQO00_RS20355 4087131..4088588 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
EQO00_RS20360 4088645..4089259 - 615 WP_000602123.1 peptide chain release factor H -
EQO00_RS20365 4089256..4090395 - 1140 WP_000521578.1 RNA ligase RtcB family protein -
EQO00_RS20370 4090585..4091037 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
EQO00_RS20375 4091047..4091445 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EQO00_RS20380 4091448..4091741 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EQO00_RS20385 4091793..4092848 - 1056 WP_001226170.1 DNA polymerase IV -
EQO00_RS20390 4092919..4093842 - 924 WP_001519504.1 putative lateral flagellar export/assembly protein LafU -
EQO00_RS20395 4093845..4094708 - 864 WP_001169519.1 flagellar motor stator protein MotA -
EQO00_RS20400 4094721..4095440 - 720 WP_000938737.1 FliA/WhiG family RNA polymerase sigma factor -
EQO00_RS20405 4095460..4095927 - 468 WP_000725264.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T118003 WP_001263501.1 NZ_CP035498:c4091445-4091047 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T118003 NZ_CP035498:c4091445-4091047 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT118003 WP_000554755.1 NZ_CP035498:c4091741-4091448 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT118003 NZ_CP035498:c4091741-4091448 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References