Detailed information of TA system TA117980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2984933..2985503
• Replicon: chromosome
• Accession: NZ_CP035494
• Organism: Microbacterium protaetiae strain DFW100M-13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ET475_RS13825
• Protein ID: WP_129391447.1
• Coordinates: 2984933..2985214 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ET475_RS13830
• Protein ID: WP_129391450.1
• Coordinates: 2985201..2985503 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ET475_RS13800	2980774..2981376	-	603	WP_129391432.1	DNA polymerase III subunit epsilon	-
ET475_RS13805	2981456..2982205	+	750	WP_129391435.1	class I SAM-dependent methyltransferase	-
ET475_RS13810	2982244..2983314	+	1071	WP_129391438.1	redox-regulated ATPase YchF	-
ET475_RS13815	2983338..2984141	+	804	WP_129391441.1	DUF2971 domain-containing protein	-
ET475_RS13820	2984251..2984817	-	567	WP_129391444.1	hypothetical protein	-
ET475_RS13825	2984933..2985214	+	282	WP_129391447.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ET475_RS13830	2985201..2985503	+	303	WP_129391450.1	HigA family addiction module antidote protein	Antitoxin
ET475_RS13835	2985551..2985955	+	405	WP_129391453.1	NUDIX domain-containing protein	-
ET475_RS13840	2986024..2986926	-	903	WP_129389508.1	IS982 family transposase	-
ET475_RS13845	2987034..2987423	-	390	WP_165310940.1	VOC family protein	-
ET475_RS13850	2987706..2988062	-	357	WP_129391459.1	hypothetical protein	-
ET475_RS13855	2988059..2989330	-	1272	WP_207205355.1	RNA polymerase sigma factor	-
ET475_RS13860	2989367..2989552	-	186	WP_129391466.1	hypothetical protein	-
ET475_RS13865	2989704..2989892	-	189	WP_129391468.1	hypothetical protein	-
ET475_RS13870	2989882..2990289	-	408	WP_129391471.1	arsenate reductase ArsC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2986024..2986926	902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10885.39 Da
• Isoelectric Point: 10.5605

>T117980 WP_129391447.1 NZ_CP035494:2984933-2985214 [Microbacterium protaetiae]
VIRSFGSKETERLWRRERVRSIDPGIHRVALRKLRQVGSAESLDDLRVPSGNRLEALKRDRVGQHGIRINDQWRICFVWT
PAGPEEVEIVDYH

Nucleotide

• Length: 282 bp

>T117980 NZ_CP035494:2984933-2985214 [Microbacterium protaetiae]
GTGATCAGATCGTTCGGTAGCAAGGAAACCGAACGGCTGTGGCGACGCGAACGTGTGCGCTCGATCGATCCGGGGATCCA
TCGGGTCGCGCTGCGCAAGTTGCGTCAGGTCGGATCCGCTGAGTCGCTCGATGACCTCCGCGTCCCATCCGGCAACCGGC
TCGAAGCGCTGAAACGTGACAGGGTTGGCCAGCACGGCATCAGGATCAACGACCAGTGGCGGATCTGCTTCGTGTGGACG
CCCGCCGGACCGGAGGAGGTGGAGATCGTTGACTATCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11256.04 Da
• Isoelectric Point: 5.1255

>AT117980 WP_129391450.1 NZ_CP035494:2985201-2985503 [Microbacterium protaetiae]
MTITDKIPPIHPGEVLMEDFIEGFEITQNKLAVAIGVPPRRINEIVHGKRGITADTALRLERYFGVSAQFWLNLQTRYEL
DLAEDRIAEQIAAITPLKVA

Nucleotide

• Length: 303 bp

>AT117980 NZ_CP035494:2985201-2985503 [Microbacterium protaetiae]
TTGACTATCACTGACAAGATTCCCCCGATTCACCCGGGCGAGGTCCTCATGGAAGATTTCATTGAGGGCTTCGAGATTAC
TCAGAACAAGCTCGCGGTCGCCATCGGCGTGCCGCCACGCCGCATCAACGAGATCGTGCACGGTAAGCGCGGGATCACGG
CCGATACGGCACTCCGTTTGGAGCGCTACTTCGGTGTCTCGGCGCAGTTTTGGCTCAACCTGCAGACGCGCTACGAGTTG
GACCTTGCCGAGGATCGGATCGCCGAGCAGATCGCCGCGATCACCCCGCTGAAGGTTGCGTGA