Detailed information of TA system TA117977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1335971..1336479
• Replicon: chromosome
• Accession: NZ_CP035494
• Organism: Microbacterium protaetiae strain DFW100M-13

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: ET475_RS06205
• Protein ID: WP_129393733.1
• Coordinates: 1335971..1336228 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: ET475_RS06210
• Protein ID: WP_129387308.1
• Coordinates: 1336228..1336479 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ET475_RS06175	1331146..1331718	+	573	WP_129387293.1	PadR family transcriptional regulator	-
ET475_RS06180	1331723..1333462	+	1740	WP_129387296.1	AarF/ABC1/UbiB kinase family protein	-
ET475_RS06185	1333473..1333988	-	516	WP_129387299.1	hypothetical protein	-
ET475_RS06190	1334161..1334349	+	189	WP_129387302.1	DUF1918 domain-containing protein	-
ET475_RS06195	1334351..1334794	-	444	WP_129387305.1	hypothetical protein	-
ET475_RS06200	1334803..1335756	-	954	WP_129393731.1	hypothetical protein	-
ET475_RS06205	1335971..1336228	-	258	WP_129393733.1	Txe/YoeB family addiction module toxin	Toxin
ET475_RS06210	1336228..1336479	-	252	WP_129387308.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
ET475_RS06215	1336539..1337225	-	687	WP_129387311.1	DUF4192 family protein	-
ET475_RS06220	1337318..1337596	-	279	WP_129387314.1	antibiotic biosynthesis monooxygenase	-
ET475_RS06225	1337645..1338283	-	639	WP_129387317.1	NADPH-dependent F420 reductase	-
ET475_RS06230	1338375..1338878	+	504	WP_165310783.1	MarR family winged helix-turn-helix transcriptional regulator	-
ET475_RS06235	1338903..1339319	+	417	WP_129387325.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
ET475_RS06240	1339326..1340255	-	930	WP_129387328.1	VOC family protein	-
ET475_RS06245	1340357..1340719	+	363	WP_129387331.1	hypothetical protein	-
ET475_RS06250	1340751..1341233	+	483	WP_129387334.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10133.73 Da
• Isoelectric Point: 10.0968

>T117977 WP_129393733.1 NZ_CP035494:c1336228-1335971 [Microbacterium protaetiae]
MRELTFDPHGWADYVAWQREDRKTLKRINALIADVLRDPFTGLGKPEPLRHVLVGAWSRRIDGKNRLVYYVDDKRIIVLK
CRDHY

Nucleotide

• Length: 258 bp

>T117977 NZ_CP035494:c1336228-1335971 [Microbacterium protaetiae]
ATGCGTGAACTCACCTTCGATCCTCACGGATGGGCGGACTATGTCGCCTGGCAGAGGGAAGATCGGAAGACGCTAAAGCG
GATCAACGCCCTGATCGCCGATGTTCTTCGCGACCCCTTCACCGGACTTGGCAAGCCGGAGCCGTTGAGACATGTCCTTG
TCGGCGCATGGTCACGGCGTATCGACGGAAAGAACCGGCTTGTGTACTACGTCGACGACAAGCGGATCATCGTCCTGAAG
TGCCGCGATCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9386.55 Da
• Isoelectric Point: 4.6509

>AT117977 WP_129387308.1 NZ_CP035494:c1336479-1336228 [Microbacterium protaetiae]
MAAITVTDARKRLFSLIQQVNDDCDVVEIVSKHGNAVLMSKDEYDSIAETAYLLSNPVDAKRLLEATERARRGEYEHHDL
IEP

Nucleotide

• Length: 252 bp

>AT117977 NZ_CP035494:c1336479-1336228 [Microbacterium protaetiae]
ATGGCTGCAATCACGGTGACGGACGCCCGCAAACGCCTGTTCAGCCTCATCCAGCAGGTCAACGATGACTGCGATGTGGT
CGAGATCGTGTCGAAGCACGGCAATGCGGTGCTCATGTCGAAGGACGAGTACGATTCCATCGCCGAGACCGCGTATCTGT
TGAGCAATCCCGTCGATGCGAAGCGGCTGCTCGAAGCGACCGAGCGCGCACGGCGGGGTGAGTACGAGCATCACGACCTC
ATTGAGCCGTGA