Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2336920..2337422 Replicon chromosome
Accession NZ_CP035486
Organism Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag EUJ32_RS11670 Protein ID WP_000767822.1
Coordinates 2336920..2337174 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EUJ32_RS11675 Protein ID WP_001259255.1
Coordinates 2337171..2337422 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUJ32_RS11650 2333413..2334771 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
EUJ32_RS11655 2334761..2334823 - 63 WP_010723108.1 membrane protein YoeI -
EUJ32_RS11660 2335038..2335967 - 930 WP_000803366.1 LysR family transcriptional regulator -
EUJ32_RS11665 2336013..2336837 - 825 WP_000754733.1 SDR family oxidoreductase -
EUJ32_RS11670 2336920..2337174 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EUJ32_RS11675 2337171..2337422 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EUJ32_RS11680 2337705..2337755 + 51 WP_001364200.1 his operon leader peptide -
EUJ32_RS11685 2337901..2338800 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EUJ32_RS11690 2338806..2340110 + 1305 WP_001297912.1 histidinol dehydrogenase -
EUJ32_RS11695 2340107..2341177 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
EUJ32_RS11700 2341177..2342244 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T117960 WP_000767822.1 NZ_CP035486:c2337174-2336920 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T117960 NZ_CP035486:c2337174-2336920 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT117960 WP_001259255.1 NZ_CP035486:c2337422-2337171 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT117960 NZ_CP035486:c2337422-2337171 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References