117949

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1665883..1666445 Replicon chromosome
Accession NZ_CP035486
Organism Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag EUJ32_RS08290 Protein ID WP_000605675.1
Coordinates 1666167..1666445 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag EUJ32_RS08285 Protein ID WP_000781370.1
Coordinates 1665883..1666167 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUJ32_RS08270 1661064..1664111 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
EUJ32_RS08275 1664124..1665008 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
EUJ32_RS08280 1665001..1665654 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
EUJ32_RS08285 1665883..1666167 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
EUJ32_RS08290 1666167..1666445 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EUJ32_RS08295 1666631..1667641 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
EUJ32_RS08300 1667775..1669472 - 1698 WP_000433460.1 malate dehydrogenase -
EUJ32_RS08305 1669629..1669766 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
EUJ32_RS08310 1669868..1670083 - 216 WP_000495766.1 biofilm-dependent modulation protein -
EUJ32_RS08315 1670428..1670859 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T117949 WP_000605675.1 NZ_CP035486:c1666445-1666167 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T117949 NZ_CP035486:c1666445-1666167 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT117949 WP_000781370.1 NZ_CP035486:c1666167-1665883 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT117949 NZ_CP035486:c1666167-1665883 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References