Detailed information of TA system TA117933

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47438..47680
• Replicon: plasmid pU13A_A
• Accession: NZ_CP035478
• Organism: Escherichia coli strain U13A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: U13A_RS25515
• Protein ID: WP_001312861.1
• Coordinates: 47438..47596 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47640..47680 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U13A_RS25470	42550..42777	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
U13A_RS25475	42865..43542	-	678	WP_001348626.1	PAS domain-containing protein	-
U13A_RS25480	43676..44059	-	384	WP_001151566.1	relaxosome protein TraM	-
U13A_RS25485	44390..44992	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
U13A_RS25490	45289..46110	-	822	WP_001234469.1	DUF945 domain-containing protein	-
U13A_RS25495	46229..46516	-	288	WP_000107535.1	hypothetical protein	-
U13A_RS27170	46541..46747	-	207	WP_000275859.1	hypothetical protein	-
U13A_RS27175	46660..46995	-	336	WP_013023876.1	hypothetical protein	-
U13A_RS25515	47438..47596	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	47640..47680	-	41	NuclAT_1	-	Antitoxin
-	47640..47680	-	41	NuclAT_1	-	Antitoxin
-	47640..47680	-	41	NuclAT_1	-	Antitoxin
-	47640..47680	-	41	NuclAT_1	-	Antitoxin
-	49124..49310	-	187	NuclAT_0	-	-
-	49124..49310	-	187	NuclAT_0	-	-
-	49124..49310	-	187	NuclAT_0	-	-
-	49124..49310	-	187	NuclAT_0	-	-
U13A_RS25525	49322..50041	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
U13A_RS25530	50038..50472	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
U13A_RS25535	50527..52485	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B	senB	1..149312	149312

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T117933 WP_001312861.1 NZ_CP035478:c47596-47438 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T117933 NZ_CP035478:c47596-47438 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT117933 NZ_CP035478:c47680-47640 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin