Detailed information of TA system TA117886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2248841..2249343
• Replicon: chromosome
• Accession: NZ_CP035476
• Organism: Escherichia coli strain U12A

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: U12A_RS14095
• Protein ID: WP_000767819.1
• Coordinates: 2248841..2249095 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: U12A_RS14100
• Protein ID: WP_001259253.1
• Coordinates: 2249092..2249343 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U12A_RS14065	2244659..2245117	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
U12A_RS14075	2245334..2246692	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
U12A_RS14080	2246682..2246744	-	63	WP_010723108.1	membrane protein YoeI	-
U12A_RS14085	2246959..2247888	-	930	WP_000803366.1	LysR family transcriptional regulator	-
U12A_RS14090	2247934..2248758	-	825	WP_000754737.1	SDR family oxidoreductase	-
U12A_RS14095	2248841..2249095	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
U12A_RS14100	2249092..2249343	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
U12A_RS14110	2249626..2249676	+	51	WP_001364200.1	his operon leader peptide	-
U12A_RS14115	2249822..2250721	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
U12A_RS14120	2250727..2252031	+	1305	WP_001296211.1	histidinol dehydrogenase	-
U12A_RS14125	2252028..2253098	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
U12A_RS14130	2253098..2254165	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2244659..2245117	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T117886 WP_000767819.1 NZ_CP035476:c2249095-2248841 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T117886 NZ_CP035476:c2249095-2248841 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT117886 WP_001259253.1 NZ_CP035476:c2249343-2249092 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT117886 NZ_CP035476:c2249343-2249092 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3