Detailed information of TA system TA117864

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32221..32491
• Replicon: plasmid pU12A_C
• Accession: NZ_CP035470
• Organism: Escherichia coli strain U12A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: U12A_RS01890
• Protein ID: WP_001312861.1
• Coordinates: 32333..32491 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32221..32284 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U12A_RS01865	27991..28530	+	540	WP_001530505.1	single-stranded DNA-binding protein	-
U12A_RS01870	28592..28825	+	234	WP_000006030.1	DUF905 family protein	-
U12A_RS01875	28890..30848	+	1959	WP_000117213.1	ParB/RepB/Spo0J family partition protein	-
U12A_RS01880	30903..31337	+	435	WP_000845910.1	conjugation system SOS inhibitor PsiB	-
U12A_RS01885	31334..32053	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
U12A_RS27490	32065..32253	-	189	WP_001336239.1	hypothetical protein	-
-	32065..32289	+	225	NuclAT_0	-	-
-	32065..32289	+	225	NuclAT_0	-	-
-	32065..32289	+	225	NuclAT_0	-	-
-	32065..32289	+	225	NuclAT_0	-	-
-	32221..32284	-	64	-	-	Antitoxin
U12A_RS01890	32333..32491	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
U12A_RS01905	33418..33705	+	288	WP_000107526.1	hypothetical protein	-
U12A_RS01910	33824..34645	+	822	WP_001234469.1	DUF945 domain-containing protein	-
U12A_RS01920	34940..35542	-	603	WP_023908348.1	transglycosylase SLT domain-containing protein	-
U12A_RS01930	35865..36248	+	384	WP_001354030.1	relaxosome protein TraM	-
U12A_RS01935	36442..37113	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
U12A_RS01940	37250..37477	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..69518	69518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T117864 WP_001312861.1 NZ_CP035470:32333-32491 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T117864 NZ_CP035470:32333-32491 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT117864 NZ_CP035470:c32284-32221 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin