Detailed information of TA system TA117854

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47443..47685
• Replicon: plasmid pU12A_A
• Accession: NZ_CP035468
• Organism: Escherichia coli strain U12A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: U12A_RS00305
• Protein ID: WP_001312861.1
• Coordinates: 47443..47601 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47645..47685 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U12A_RS00260	42555..42782	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
U12A_RS00265	42870..43547	-	678	WP_001348626.1	PAS domain-containing protein	-
U12A_RS00270	43681..44064	-	384	WP_001151566.1	relaxosome protein TraM	-
U12A_RS00275	44395..44997	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
U12A_RS00280	45294..46115	-	822	WP_001234469.1	DUF945 domain-containing protein	-
U12A_RS00285	46234..46521	-	288	WP_000107535.1	hypothetical protein	-
U12A_RS27655	46546..46752	-	207	WP_000275859.1	hypothetical protein	-
U12A_RS27660	46665..47000	-	336	WP_013023876.1	hypothetical protein	-
U12A_RS00305	47443..47601	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	47645..47685	-	41	NuclAT_1	-	Antitoxin
-	47645..47685	-	41	NuclAT_1	-	Antitoxin
-	47645..47685	-	41	NuclAT_1	-	Antitoxin
-	47645..47685	-	41	NuclAT_1	-	Antitoxin
-	49129..49315	-	187	NuclAT_0	-	-
-	49129..49315	-	187	NuclAT_0	-	-
-	49129..49315	-	187	NuclAT_0	-	-
-	49129..49315	-	187	NuclAT_0	-	-
U12A_RS00315	49327..50046	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
U12A_RS00320	50043..50477	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
U12A_RS00325	50532..52490	-	1959	WP_129765214.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / blaTEM-1B	senB	1..151747	151747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T117854 WP_001312861.1 NZ_CP035468:c47601-47443 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T117854 NZ_CP035468:c47601-47443 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT117854 NZ_CP035468:c47685-47645 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin