Detailed information of TA system TA117850

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5633..5872
• Replicon: plasmid pU12A_A
• Accession: NZ_CP035468
• Organism: Escherichia coli strain U12A

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: U12A_RS00055
• Protein ID: WP_023144756.1
• Coordinates: 5633..5767 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	5812..5872 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U12A_RS00015	1644..2045	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
U12A_RS00020	1978..2235	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
U12A_RS00025	2328..2981	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
U12A_RS00035	3921..4778	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
U12A_RS00040	4771..4845	-	75	WP_031943482.1	RepA leader peptide Tap	-
U12A_RS27650	4842..4976	-	135	Protein_7	protein CopA/IncA	-
U12A_RS00050	5082..5336	-	255	WP_000083850.1	replication regulatory protein RepA	-
U12A_RS00055	5633..5767	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	5812..5872	+	61	NuclAT_2	-	Antitoxin
-	5812..5872	+	61	NuclAT_2	-	Antitoxin
-	5812..5872	+	61	NuclAT_2	-	Antitoxin
-	5812..5872	+	61	NuclAT_2	-	Antitoxin
U12A_RS27465	5839..6125	-	287	Protein_10	DUF2726 domain-containing protein	-
U12A_RS00065	6203..7816	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
U12A_RS00070	7847..8197	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
U12A_RS00075	8194..8619	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
U12A_RS00085	9178..9390	-	213	WP_013023861.1	hypothetical protein	-
U12A_RS00090	9521..10081	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / blaTEM-1B	senB	1..151747	151747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T117850 WP_023144756.1 NZ_CP035468:c5767-5633 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T117850 NZ_CP035468:c5767-5633 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT117850 NZ_CP035468:5812-5872 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin