Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4494051..4494682 Replicon chromosome
Accession NZ_CP035467
Organism Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EQU24_RS20000 Protein ID WP_017841861.1
Coordinates 4494051..4494377 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EQU24_RS20005 Protein ID WP_014150329.1
Coordinates 4494392..4494682 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQU24_RS19970 4489397..4490098 + 702 WP_017841866.1 urate hydroxylase PuuD -
EQU24_RS19975 4490396..4490947 + 552 WP_014150334.1 hypothetical protein -
EQU24_RS22605 4491703..4491846 + 144 WP_017841865.1 hypothetical protein -
EQU24_RS19985 4492435..4493160 + 726 WP_017841863.1 DUF429 domain-containing protein -
EQU24_RS19995 4493621..4493854 + 234 WP_017841862.1 addiction module protein -
EQU24_RS20000 4494051..4494377 + 327 WP_017841861.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EQU24_RS20005 4494392..4494682 + 291 WP_014150329.1 HigA family addiction module antidote protein Antitoxin
EQU24_RS20010 4495074..4495343 + 270 WP_017841860.1 BrnT family toxin -
EQU24_RS20015 4495333..4495533 + 201 WP_014150327.1 hypothetical protein -
EQU24_RS20020 4495813..4497075 - 1263 WP_040575839.1 FAD-binding oxidoreductase -
EQU24_RS20025 4497148..4497828 - 681 WP_017841857.1 SDR family oxidoreductase -
EQU24_RS20035 4497980..4498567 - 588 WP_017841856.1 hypothetical protein -
EQU24_RS20040 4499079..4499480 + 402 WP_017841855.1 TIGR03790 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4371035..4657012 285977


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(16-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-69)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12355.08 Da        Isoelectric Point: 7.0605

>T117845 WP_017841861.1 NZ_CP035467:4494051-4494377 [Methylotuvimicrobium buryatense]
MHEYRESVNIGIMIVSFGDKATSDLFHGISSNKARKLPNQIHELALYKLDIINAAQNLDDLKSPPGNRLEPLKGDLKGFH
SIRINNQWCIVFRWEANAAHDVQIMDYH

Download         Length: 327 bp

>T117845 NZ_CP035467:4494051-4494377 [Methylotuvimicrobium buryatense]
TTGCATGAATACCGTGAATCCGTTAATATCGGTATCATGATTGTTTCATTCGGTGATAAGGCCACTTCAGATTTGTTTCA
CGGAATTTCCAGCAACAAAGCTAGAAAACTGCCCAACCAGATTCATGAATTGGCGCTGTATAAGCTGGACATAATCAATG
CTGCTCAAAACCTTGACGATCTTAAATCTCCGCCTGGAAACAGACTGGAACCACTCAAAGGTGATCTCAAGGGTTTTCAC
AGTATCCGTATAAACAATCAATGGTGCATTGTTTTCCGTTGGGAGGCCAATGCAGCTCATGATGTCCAAATTATGGACTA
TCACTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10709.27 Da        Isoelectric Point: 6.6420

>AT117845 WP_014150329.1 NZ_CP035467:4494392-4494682 [Methylotuvimicrobium buryatense]
MIPTNRTATHPGTILLKEFLEPLEITQKALAIHLGISVQRVNEIVRGKRGITPDTAWLLSEAFNTSPEFWLNLQSTHDLS
AHRPEYHVESLVAAGI

Download         Length: 291 bp

>AT117845 NZ_CP035467:4494392-4494682 [Methylotuvimicrobium buryatense]
ATGATTCCGACGAACAGAACTGCAACACACCCAGGTACGATATTACTTAAAGAGTTTCTTGAGCCTCTTGAGATTACCCA
AAAAGCCCTGGCTATTCATTTAGGTATATCCGTTCAACGCGTTAATGAGATTGTACGTGGTAAACGTGGTATTACGCCAG
ATACTGCCTGGCTACTTTCTGAAGCTTTCAATACGTCCCCTGAATTTTGGCTTAATCTGCAATCGACTCATGATCTGTCG
GCTCATAGACCTGAGTATCATGTCGAGTCTTTGGTTGCTGCGGGTATATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References