Detailed information of TA system TA117844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4383889..4384482
• Replicon: chromosome
• Accession: NZ_CP035467
• Organism: Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4P9URQ5
• Locus tag: EQU24_RS19525
• Protein ID: WP_026130311.1
• Coordinates: 4383889..4384167 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EQU24_RS19530
• Protein ID: WP_014146975.1
• Coordinates: 4384180..4384482 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQU24_RS19515	4379235..4379630	-	396	WP_017841948.1	hypothetical protein	-
EQU24_RS19520	4381328..4382239	-	912	WP_017841947.1	type II secretion system protein	-
EQU24_RS19525	4383889..4384167	+	279	WP_026130311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EQU24_RS19530	4384180..4384482	+	303	WP_014146975.1	HigA family addiction module antidote protein	Antitoxin
EQU24_RS19535	4384576..4384902	-	327	WP_017841945.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EQU24_RS19540	4384902..4385183	-	282	WP_017841944.1	type II toxin-antitoxin system ParD family antitoxin	-
EQU24_RS19545	4385317..4385739	-	423	WP_040575345.1	hypothetical protein	-
EQU24_RS19550	4385830..4387110	-	1281	WP_017841942.1	hypothetical protein	-
EQU24_RS19555	4387408..4388346	-	939	WP_014146970.1	Nudix family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4371035..4657012	285977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10594.04 Da
• Isoelectric Point: 9.7588

>T117844 WP_026130311.1 NZ_CP035467:4383889-4384167 [Methylotuvimicrobium buryatense]
MIKSFHCKDTQALFEGESPKRFRAFQAIAERKLAQLHAAASLAFLRSPPGNRLEALKGSRAGQYSIRINDQWRVGFRWLD
NDAFEVEIVDYH

Nucleotide

• Length: 279 bp

>T117844 NZ_CP035467:4383889-4384167 [Methylotuvimicrobium buryatense]
ATGATTAAGTCGTTTCACTGCAAAGACACCCAAGCCTTGTTCGAAGGAGAATCGCCTAAACGTTTTCGCGCGTTTCAAGC
TATTGCCGAACGTAAGTTGGCGCAACTGCATGCCGCGGCTAGCCTGGCGTTTTTACGCAGCCCTCCGGGCAATCGCCTGG
AAGCGCTAAAAGGGAGTCGTGCGGGACAATACAGCATTCGCATCAATGACCAGTGGAGAGTGGGTTTTCGCTGGTTGGAT
AACGATGCTTTTGAGGTTGAAATTGTTGATTACCATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11356.99 Da
• Isoelectric Point: 7.3198

>AT117844 WP_014146975.1 NZ_CP035467:4384180-4384482 [Methylotuvimicrobium buryatense]
MIENRMRPIHPGEILREDFLNPLALSVNGLATALHVPTTRLHEIVKERRAITPDTALRLARYFGGDAQSWLNLQTAYDLR
RAQQSSLDAIEREVLPREAA

Nucleotide

• Length: 303 bp

>AT117844 NZ_CP035467:4384180-4384482 [Methylotuvimicrobium buryatense]
ATGATAGAAAACCGTATGCGTCCGATACACCCCGGCGAAATCCTGCGTGAAGATTTTTTGAATCCCTTGGCATTAAGCGT
AAACGGGTTGGCAACCGCTTTGCACGTTCCGACCACGCGTTTGCATGAAATCGTTAAGGAACGCCGCGCGATTACGCCGG
ATACCGCTTTGCGCTTGGCGCGTTATTTTGGCGGAGATGCACAATCCTGGCTGAACCTGCAAACAGCTTATGACTTACGC
CGGGCACAGCAATCCTCGCTTGACGCCATAGAGCGCGAGGTTTTGCCGCGCGAGGCGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A4P9URQ5