Detailed information of TA system TA117843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4113140..4113738
• Replicon: chromosome
• Accession: NZ_CP035467
• Organism: Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4P9UTQ2
• Locus tag: EQU24_RS18440
• Protein ID: WP_017842147.1
• Coordinates: 4113415..4113738 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EQU24_RS18435
• Protein ID: WP_017842148.1
• Coordinates: 4113140..4113418 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQU24_RS18400	4108220..4108906	-	687	WP_017842154.1	transposase	-
EQU24_RS18405	4108911..4109351	-	441	WP_017842153.1	type II toxin-antitoxin system VapC family toxin	-
EQU24_RS18410	4109351..4109584	-	234	WP_017842152.1	DUF2191 domain-containing protein	-
EQU24_RS18415	4109740..4110024	-	285	WP_017842151.1	putative addiction module antidote protein	-
EQU24_RS18420	4110035..4110328	-	294	WP_017842150.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EQU24_RS18425	4110464..4111021	+	558	WP_138767217.1	transposase	-
EQU24_RS18430	4111417..4112844	+	1428	WP_175421704.1	IS4 family transposase	-
EQU24_RS18435	4113140..4113418	-	279	WP_017842148.1	helix-turn-helix domain-containing protein	Antitoxin
EQU24_RS18440	4113415..4113738	-	324	WP_017842147.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EQU24_RS18445	4114303..4115189	+	887	Protein_3483	dTDP-4-dehydrorhamnose reductase	-
EQU24_RS18450	4115186..4116073	+	888	WP_017842146.1	glucose-1-phosphate thymidylyltransferase RfbA	-
EQU24_RS18455	4116087..4116632	+	546	WP_017842145.1	dTDP-4-dehydrorhamnose 3,5-epimerase	-
EQU24_RS18465	4116973..4117650	+	678	WP_017842144.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-85)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12492.64 Da
• Isoelectric Point: 9.8098

>T117843 WP_017842147.1 NZ_CP035467:c4113738-4113415 [Methylotuvimicrobium buryatense]
MIWKIDFYGDVEERILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKSKHIYVLH
VFVKKSQKTPRNELELARERQREVQKS

Nucleotide

• Length: 324 bp

>T117843 NZ_CP035467:c4113738-4113415 [Methylotuvimicrobium buryatense]
ATGATCTGGAAAATCGACTTTTATGGTGATGTAGAGGAGCGAATCCTTGATATGCCACCTAAAATCCAAGCACGGATGAT
TAAGCTTTTAGAGTTAATGGAAAAGCACGGTGCTAACTTGGGACCACCACACACGGAGTCAATGGGTGACGGCCTATTTG
AAATAAGAGCTAAGGCTCAAGAAGGAATTGGGCGTGGGTTATTTTGTTACTTGAAAAGCAAGCATATCTATGTGCTGCAT
GTTTTTGTAAAAAAATCTCAAAAGACGCCAAGAAATGAGCTCGAACTGGCTAGAGAACGACAAAGAGAGGTTCAAAAATC
ATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10494.10 Da
• Isoelectric Point: 9.9392

>AT117843 WP_017842148.1 NZ_CP035467:c4113418-4113140 [Methylotuvimicrobium buryatense]
MSLQKLKQRALTNPDVKAEYEKLESEFNLIDQLLSMRTKAGLTQEQVAERMQTQKSNISRLEKGNANPSWSTLLKYAHAC
GFELSLKPQKIV

Nucleotide

• Length: 279 bp

>AT117843 NZ_CP035467:c4113418-4113140 [Methylotuvimicrobium buryatense]
ATGAGTCTTCAAAAACTGAAACAACGCGCTTTAACGAACCCTGATGTTAAAGCTGAATATGAAAAACTTGAAAGTGAGTT
CAATCTCATTGATCAGTTGCTCTCAATGCGCACTAAAGCTGGTCTGACTCAAGAGCAGGTTGCAGAGAGAATGCAGACCC
AAAAGAGTAACATTAGCCGCTTAGAAAAAGGAAATGCCAATCCTAGCTGGTCAACGCTTCTCAAGTATGCCCATGCCTGT
GGGTTTGAGCTTTCCCTTAAACCGCAGAAAATAGTTTAA

Structures

Toxin

• AlphaFold DB: A0A4P9UTQ2