Detailed information of TA system TA117812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 143312..143822
• Replicon: chromosome
• Accession: NZ_CP035458
• Organism: Photobacterium damselae subsp. damselae strain KC-Na-NB1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: ETN89_RS15345
• Protein ID: WP_086368444.1
• Coordinates: 143562..143822 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A2T3QIT0
• Locus tag: ETN89_RS15340
• Protein ID: WP_036765838.1
• Coordinates: 143312..143572 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETN89_RS15315	138798..139397	+	600	WP_065171320.1	LemA family protein	-
ETN89_RS15320	139398..140153	+	756	WP_086368447.1	TPM domain-containing protein	-
ETN89_RS15325	140156..140773	+	618	WP_086368446.1	TPM domain-containing protein	-
ETN89_RS15330	140837..141361	+	525	WP_157756344.1	hypothetical protein	-
ETN89_RS15335	141436..143118	+	1683	WP_086368560.1	RluA family pseudouridine synthase	-
ETN89_RS15340	143312..143572	+	261	WP_036765838.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ETN89_RS15345	143562..143822	+	261	WP_086368444.1	Txe/YoeB family addiction module toxin	Toxin
ETN89_RS15350	143862..144116	-	255	WP_086368443.1	hypothetical protein	-
ETN89_RS15355	144128..145012	-	885	WP_086368442.1	AraC family transcriptional regulator	-
ETN89_RS15360	145172..146320	+	1149	WP_086368441.1	iron-containing alcohol dehydrogenase	-
ETN89_RS15365	146505..147002	+	498	WP_129382733.1	hypothetical protein	-
ETN89_RS15370	147035..148066	+	1032	WP_086367099.1	IS630 family transposase	-
ETN89_RS15375	148054..148440	+	387	WP_086368439.1	HNH endonuclease	-
ETN89_RS15380	148502..148813	-	312	WP_157756343.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10318.87 Da
• Isoelectric Point: 9.9416

>T117812 WP_086368444.1 NZ_CP035458:143562-143822 [Photobacterium damselae subsp. damselae]
MMINFTPNAWDDYLYWQSQDKKTLKRINKLIENCVRTPFEGIGKPEPLKDNLSGYWSRRIDDTHRLVYKATQNTLVIVAV
RFHYQK

Nucleotide

• Length: 261 bp

>T117812 NZ_CP035458:143562-143822 [Photobacterium damselae subsp. damselae]
ATGATGATTAATTTCACGCCTAACGCTTGGGATGATTACTTATACTGGCAATCTCAAGATAAGAAGACTTTAAAACGAAT
CAATAAACTGATTGAGAATTGTGTTAGAACGCCGTTCGAAGGAATTGGAAAGCCTGAACCATTGAAAGATAACTTATCAG
GCTATTGGTCGCGACGCATTGATGATACACATAGATTAGTCTATAAAGCCACTCAAAATACCTTAGTCATTGTAGCTGTG
CGGTTTCACTACCAAAAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9718.89 Da
• Isoelectric Point: 4.3167

>AT117812 WP_036765838.1 NZ_CP035458:143312-143572 [Photobacterium damselae subsp. damselae]
MTVLSFSEVRGNFKKVCDQVVDDCDAVVIHRRDADNVVLMSESEFNSWKETMYLMSNPVNAANLLESIKQAECGNVQTHE
LLSHDD

Nucleotide

• Length: 261 bp

>AT117812 NZ_CP035458:143312-143572 [Photobacterium damselae subsp. damselae]
ATGACCGTTTTGAGTTTTTCTGAAGTTCGAGGCAATTTTAAAAAAGTATGCGATCAAGTCGTTGATGATTGCGATGCAGT
AGTTATCCATCGACGTGATGCAGATAATGTCGTGCTTATGTCTGAATCTGAGTTTAATAGTTGGAAGGAAACGATGTATC
TCATGTCTAATCCTGTAAATGCTGCAAACTTACTTGAATCAATAAAACAAGCTGAGTGTGGTAATGTACAGACACATGAG
CTACTAAGCCATGATGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A2T3QIT0