Detailed information of TA system TA117717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1174804..1175531
• Replicon: chromosome
• Accession: NZ_CP035385
• Organism: Enterobacter hormaechei strain S11_16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EQ802_RS05580
• Protein ID: WP_063147977.1
• Coordinates: 1175220..1175531 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EQ802_RS05575
• Protein ID: WP_063147976.1
• Coordinates: 1174804..1175223 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQ802_RS05540	1170660..1170962	+	303	WP_063147972.1	hypothetical protein	-
EQ802_RS05545	1171048..1171493	+	446	Protein_1065	hypothetical protein	-
EQ802_RS05550	1171505..1172038	+	534	Protein_1066	nuclease	-
EQ802_RS05555	1172246..1172650	+	405	WP_063147973.1	SMI1/KNR4 family protein	-
EQ802_RS05560	1173007..1173387	+	381	WP_045309197.1	hypothetical protein	-
EQ802_RS05565	1173476..1174090	+	615	WP_063147974.1	hypothetical protein	-
EQ802_RS05570	1174090..1174536	+	447	WP_063147975.1	hypothetical protein	-
EQ802_RS05575	1174804..1175223	-	420	WP_063147976.1	helix-turn-helix domain-containing protein	Antitoxin
EQ802_RS05580	1175220..1175531	-	312	WP_063147977.1	type II toxin-antitoxin system HigB family toxin	Toxin
EQ802_RS05585	1175752..1176162	-	411	WP_023299534.1	ASCH domain-containing protein	-
EQ802_RS05590	1176363..1176818	-	456	WP_063147978.1	hypothetical protein	-
EQ802_RS05595	1176845..1176952	-	108	WP_157843337.1	ash family protein	-
EQ802_RS05605	1177669..1177881	+	213	WP_022647355.1	RNA chaperone/antiterminator CspA	-
EQ802_RS05610	1178109..1179005	-	897	WP_063147979.1	LysR family transcriptional regulator	-
EQ802_RS05615	1179111..1179890	+	780	WP_088581799.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12542.46 Da
• Isoelectric Point: 10.0930

>T117717 WP_063147977.1 NZ_CP035385:c1175531-1175220 [Enterobacter hormaechei]
MPAVSRAPFDAATRHFPIQELALEDLYWVLKGEHYPSPDDMRKRFPSRERMKYREKWWVIDVGGGHLRVMFFADFERRKI
FIKHISTHADYDKLTDLYRRKKE

Nucleotide

• Length: 312 bp

>T117717 NZ_CP035385:c1175531-1175220 [Enterobacter hormaechei]
ATGCCCGCAGTCTCAAGAGCGCCTTTCGACGCGGCCACAAGACATTTTCCTATTCAAGAACTCGCTCTGGAAGATCTTTA
CTGGGTACTAAAAGGAGAGCACTACCCATCACCAGACGACATGCGAAAGCGCTTCCCCAGCCGGGAGAGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATTTAAGAGTGATGTTTTTTGCTGATTTTGAGCGTAGAAAGATC
TTCATCAAGCACATCAGCACTCACGCAGATTATGACAAGCTCACGGACCTCTACCGGAGAAAGAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15507.54 Da
• Isoelectric Point: 4.7722

>AT117717 WP_063147976.1 NZ_CP035385:c1175223-1174804 [Enterobacter hormaechei]
MNYSAAIKAANDLTRELPFLGSSPSRQDYEDAMGLVEYLIEHDPDSPLVEMLTAKIDKYENESPEFAEFNARVASIPSGV
ALLRTLMDQYHLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGVPVSAFVGN

Nucleotide

• Length: 420 bp

>AT117717 NZ_CP035385:c1175223-1174804 [Enterobacter hormaechei]
ATGAACTATTCAGCAGCCATCAAAGCGGCAAACGATCTAACAAGGGAGCTCCCTTTCCTGGGCTCAAGCCCGTCGCGACA
AGATTATGAGGATGCTATGGGGTTGGTGGAGTATCTAATCGAGCATGACCCCGATAGTCCGCTGGTAGAGATGCTCACCG
CTAAAATCGATAAATACGAAAACGAGTCTCCGGAGTTTGCAGAATTTAACGCGCGTGTTGCCAGCATTCCGTCAGGCGTT
GCGTTGTTGCGAACGCTGATGGATCAGTACCATCTCACTCAGAGCGATTTCGAGAACGAGATAGGCAAGAAGTCCCTGGT
GAGTCGAATCCTCAACGGACAGCGCACGTTAACGCTGGATCATATGCGCGCGCTGGCGAAGCGTTTTGGCGTGCCGGTAA
GTGCATTTGTGGGAAATTGA