Detailed information of TA system TA117715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 30125..30855
• Replicon: chromosome
• Accession: NZ_CP035385
• Organism: Enterobacter hormaechei strain S11_16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155SSD8
• Locus tag: EQ802_RS00155
• Protein ID: WP_022646552.1
• Coordinates: 30125..30439 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EQ802_RS00160
• Protein ID: WP_023305145.1
• Coordinates: 30436..30855 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQ802_RS00130	26207..27292	+	1086	Protein_25	cellulase family glycosylhydrolase	-
EQ802_RS00135	27198..28382	-	1185	WP_023298963.1	multidrug efflux MFS transporter EmrD	-
EQ802_RS00140	28558..28975	-	418	Protein_27	EamA family transporter	-
EQ802_RS00145	29038..29484	-	447	WP_023298965.1	GNAT family N-acetyltransferase	-
EQ802_RS00150	29549..29638	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
EQ802_RS00155	30125..30439	+	315	WP_022646552.1	type II toxin-antitoxin system HigB family toxin	Toxin
EQ802_RS00160	30436..30855	+	420	WP_023305145.1	helix-turn-helix domain-containing protein	Antitoxin
EQ802_RS00165	31002..31100	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
EQ802_RS00170	31207..32895	+	1689	WP_063148199.1	acetolactate synthase large subunit	-
EQ802_RS00175	32899..33186	+	288	WP_008500144.1	acetolactate synthase small subunit	-
EQ802_RS00180	33312..33905	+	594	WP_022646555.1	transcriptional regulator UhpA	-
EQ802_RS00185	33902..35407	+	1506	WP_048966212.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.55 Da
• Isoelectric Point: 10.1989

>T117715 WP_022646552.1 NZ_CP035385:30125-30439 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQYREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T117715 NZ_CP035385:30125-30439 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15508.15 Da
• Isoelectric Point: 5.6761

>AT117715 WP_023305145.1 NZ_CP035385:30436-30855 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAVRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT117715 NZ_CP035385:30436-30855 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGTCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A155SSD8