Detailed information of TA system TA117712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 189832..190559
• Replicon: plasmid pAP855
• Accession: NZ_CP035384
• Organism: Klebsiella pneumoniae strain AP8555

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: ES964_RS27920
• Protein ID: WP_011251285.1
• Coordinates: 190248..190559 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ES964_RS27915
• Protein ID: WP_011251286.1
• Coordinates: 189832..190251 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES964_RS27880	185000..185470	-	471	WP_048333570.1	hypothetical protein	-
ES964_RS30050	185663..186418	-	756	Protein_199	hypothetical protein	-
ES964_RS27890	187014..187457	+	444	WP_048333569.1	hypothetical protein	-
ES964_RS27895	187478..188266	+	789	WP_040217257.1	hypothetical protein	-
ES964_RS27900	188280..188615	+	336	Protein_202	conjugal transfer protein TraI	-
ES964_RS27905	188717..189685	-	969	WP_074428168.1	IS5 family transposase	-
ES964_RS27915	189832..190251	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
ES964_RS27920	190248..190559	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
ES964_RS27925	190764..191222	-	459	Protein_206	DDE-type integrase/transposase/recombinase	-
ES964_RS27930	191336..192033	+	698	Protein_207	IS1 family transposase	-
ES964_RS27935	192050..192484	+	435	Protein_208	thioredoxin fold domain-containing protein	-
ES964_RS27940	192501..192812	+	312	WP_129075064.1	hypothetical protein	-
ES964_RS27945	192826..193167	+	342	WP_011251281.1	hypothetical protein	-
ES964_RS27950	193227..194183	+	957	WP_011251280.1	DsbA family protein	-
ES964_RS27955	194300..194875	+	576	WP_074424927.1	hypothetical protein	-
ES964_RS27960	194983..195498	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..357837	357837
	-	inside	IScluster/Tn	-	-	179711..192033	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T117712 WP_011251285.1 NZ_CP035384:c190559-190248 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T117712 NZ_CP035384:c190559-190248 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT117712 WP_011251286.1 NZ_CP035384:c190251-189832 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT117712 NZ_CP035384:c190251-189832 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1