Detailed information of TA system TA117673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 73896..74623
• Replicon: plasmid pLA-109
• Accession: NZ_CP035380
• Organism: Leclercia adecarboxylata strain R25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A482LZ08
• Locus tag: ES815_RS00410
• Protein ID: WP_045898923.1
• Coordinates: 73896..74207 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ES815_RS00415
• Protein ID: WP_103181184.1
• Coordinates: 74204..74623 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES815_RS00385	69242..69916	-	675	Protein_75	efflux RND transporter permease subunit	-
ES815_RS00390	69922..70059	-	138	Protein_76	hypothetical protein	-
ES815_RS00395	70104..70517	-	414	WP_103181181.1	hypothetical protein	-
ES815_RS00400	71176..71589	+	414	WP_130588776.1	hypothetical protein	-
ES815_RS00405	72449..72847	+	399	WP_103825222.1	H-NS histone family protein	-
ES815_RS00410	73896..74207	+	312	WP_045898923.1	type II toxin-antitoxin system HigB family toxin	Toxin
ES815_RS00415	74204..74623	+	420	WP_103181184.1	helix-turn-helix domain-containing protein	Antitoxin
ES815_RS00420	74601..74794	-	194	Protein_82	hypothetical protein	-
ES815_RS00425	74808..75955	-	1148	Protein_83	IS3 family transposase	-
ES815_RS23695	76581..76730	-	150	WP_158250578.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108995	108995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12347.07 Da
• Isoelectric Point: 9.7248

>T117673 WP_045898923.1 NZ_CP035380:73896-74207 [Leclercia adecarboxylata]
MHVISKEPFEEAAKQYPNDSLAVRALYRLVRETDFSSPAEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T117673 NZ_CP035380:73896-74207 [Leclercia adecarboxylata]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCAATATCCCAACGATTCGTTAGCCGTTCGGGCGCTGTA
CCGCTTAGTCCGCGAGACGGACTTTTCTTCTCCAGCTGAGATGCGAACGCTGATACCGAGCCTGGACAATTTTAAGTATC
GTAATAAGTGGTGGGTGTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTGAATAAACGCTTC
TATGTGAAGCACATCGCCACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15475.62 Da
• Isoelectric Point: 4.3573

>AT117673 WP_103181184.1 NZ_CP035380:74204-74623 [Leclercia adecarboxylata]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAIELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEMPV
GVALLRTLIDQYRLSYSDLKEEIGSKSLVSQILSGQRSLTIMHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT117673 NZ_CP035380:74204-74623 [Leclercia adecarboxylata]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCTGTTCCCTTCCTGGGGGGCAGTTCGTC
CGAGAGCGATTACCGCGAAGCGATAGAGTTGGTGGACTATCTGATCGAAAACGACGACGAGAACCCGCTTATTGACTTCC
TGGCGAGTAAAATTGCTGACTATGAGGATAACAGCCCTCGTTTCGCTGAATTCAACAAGGCTATCGCTGAAATGCCAGTC
GGCGTTGCCCTTCTGCGCACCCTGATTGACCAGTACAGGCTGTCTTATTCTGACCTTAAGGAGGAGATCGGCTCTAAATC
GCTGGTTAGCCAGATTCTCTCTGGTCAGCGGTCACTGACAATCATGCACATTAAGGCGCTGTCTGCTCGTTTTGGTGTCA
AACCAGAGTGGTTCCTTTGA

Structures

Toxin

• AlphaFold DB: A0A482LZ08