Detailed information of TA system TA117624

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1770841..1771066
• Replicon: chromosome
• Accession: NZ_CP035366
• Organism: Escherichia coli O157:H7 strain C1-057

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ES669_RS09210
• Protein ID: WP_000813254.1
• Coordinates: 1770911..1771066 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1770841..1770899 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES669_RS09165	1766094..1767047	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
ES669_RS09170	1767054..1767794	+	741	WP_000790456.1	ATP-binding protein	-
ES669_RS09175	1767824..1768594	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
ES669_RS09180	1768610..1769005	+	396	WP_001118161.1	DUF977 family protein	-
ES669_RS09185	1769062..1769418	+	357	WP_001302146.1	hypothetical protein	-
ES669_RS09190	1769467..1769679	+	213	WP_000063625.1	hypothetical protein	-
ES669_RS09195	1769715..1770086	+	372	WP_000137941.1	hypothetical protein	-
ES669_RS09200	1770083..1770445	+	363	WP_000610379.1	DUF551 domain-containing protein	-
ES669_RS09205	1770561..1770665	+	105	WP_001278450.1	hypothetical protein	-
-	1770841..1770899	-	59	-	-	Antitoxin
ES669_RS09210	1770911..1771066	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
ES669_RS28410	1771363..1771530	+	168	WP_000998188.1	hypothetical protein	-
ES669_RS09215	1771596..1771874	+	279	WP_001304183.1	hypothetical protein	-
ES669_RS09220	1771876..1772925	+	1050	Protein_1738	DUF968 domain-containing protein	-
ES669_RS09225	1772938..1773312	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
ES669_RS09230	1773309..1774130	+	822	WP_000762904.1	antitermination protein	-
ES669_RS09235	1774357..1774554	+	198	WP_000917735.1	hypothetical protein	-
ES669_RS09240	1774705..1775763	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / espM1	1697125..1806371	109246

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T117624 WP_000813254.1 NZ_CP035366:1770911-1771066 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T117624 NZ_CP035366:1770911-1771066 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT117624 NZ_CP035366:c1770899-1770841 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin