Detailed information of TA system TA117619

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1200260..1200485
• Replicon: chromosome
• Accession: NZ_CP035366
• Organism: Escherichia coli O157:H7 strain C1-057

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ES669_RS05770
• Protein ID: WP_000813263.1
• Coordinates: 1200330..1200485 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1200260..1200318 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ES669_RS05735	1195535..1196626	+	1092	WP_001205823.1	hypothetical protein	-
ES669_RS05740	1196633..1197379	+	747	WP_000788751.1	ATP-binding protein	-
ES669_RS05745	1197401..1198171	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
ES669_RS05750	1198187..1198600	+	414	WP_001151233.1	DUF977 family protein	-
ES669_RS05755	1198952..1199725	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1200260..1200318	-	59	-	-	Antitoxin
ES669_RS05770	1200330..1200485	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
ES669_RS05775	1200653..1200931	+	279	WP_001341388.1	hypothetical protein	-
ES669_RS05780	1200933..1201982	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
ES669_RS05785	1201995..1202366	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ES669_RS05790	1202356..1202727	+	372	WP_000090264.1	antitermination protein	-
ES669_RS05795	1202879..1203697	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ES669_RS05800	1203984..1204180	+	197	Protein_1107	TrmB family transcriptional regulator	-
ES669_RS05805	1204318..1205031	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T117619 WP_000813263.1 NZ_CP035366:1200330-1200485 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T117619 NZ_CP035366:1200330-1200485 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT117619 NZ_CP035366:c1200318-1200260 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin