Detailed information of TA system TA117601

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30013..30283
• Replicon: plasmid pD72-F33
• Accession: NZ_CP035314
• Organism: Escherichia coli strain D72

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ERL60_RS23050
• Protein ID: WP_001312861.1
• Coordinates: 30125..30283 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30013..30076 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERL60_RS23025	25724..26251	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
ERL60_RS23030	26309..26542	+	234	WP_000006003.1	DUF905 family protein	-
ERL60_RS23035	26603..28626	+	2024	Protein_35	ParB/RepB/Spo0J family partition protein	-
ERL60_RS23040	28695..29129	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
ERL60_RS23045	29126..29845	+	720	WP_131419573.1	plasmid SOS inhibition protein A	-
-	29857..30081	+	225	NuclAT_0	-	-
-	29857..30081	+	225	NuclAT_0	-	-
-	29857..30081	+	225	NuclAT_0	-	-
-	29857..30081	+	225	NuclAT_0	-	-
ERL60_RS24000	29866..30045	-	180	WP_001309233.1	hypothetical protein	-
-	30013..30076	-	64	-	-	Antitoxin
ERL60_RS23050	30125..30283	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ERL60_RS24075	30521..30898	-	378	Protein_40	hypothetical protein	-
ERL60_RS23070	31198..31494	+	297	WP_001272251.1	hypothetical protein	-
ERL60_RS23075	31605..32426	+	822	WP_001234445.1	DUF945 domain-containing protein	-
ERL60_RS23080	32723..33370	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
ERL60_RS23085	33647..34030	+	384	WP_000124981.1	relaxosome protein TraM	-
ERL60_RS23090	34221..34907	+	687	WP_015059009.1	PAS domain-containing protein	-
ERL60_RS23095	35001..35228	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-55	-	1..89963	89963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T117601 WP_001312861.1 NZ_CP035314:30125-30283 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T117601 NZ_CP035314:30125-30283 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT117601 NZ_CP035314:c30076-30013 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin