117599

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4100052..4100549 Replicon chromosome
Accession NZ_CP035312
Organism Escherichia coli strain D72

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ERL60_RS19970 Protein ID WP_001173343.1
Coordinates 4100052..4100225 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W4RHQ7
Locus tag ERL60_RS19975 Protein ID WP_001600874.1
Coordinates 4100253..4100549 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ERL60_RS19960 4096722..4098179 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
ERL60_RS19965 4098416..4099933 + 1518 WP_001295090.1 lysine--tRNA ligase -
ERL60_RS19970 4100052..4100225 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ERL60_RS19975 4100253..4100549 - 297 WP_001600874.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ERL60_RS19980 4100776..4101048 - 273 WP_000405651.1 N-acetyltransferase -
ERL60_RS19985 4101060..4101290 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ERL60_RS19990 4101471..4103102 + 1632 WP_001216477.1 sensor histidine kinase -
ERL60_RS19995 4103099..4103818 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4091198..4100549 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T117599 WP_001173343.1 NZ_CP035312:c4100225-4100052 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T117599 NZ_CP035312:c4100225-4100052 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.00 Da        Isoelectric Point: 4.1705

>AT117599 WP_001600874.1 NZ_CP035312:c4100549-4100253 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRTLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT117599 NZ_CP035312:c4100549-4100253 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGAACATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W4RHQ7

References