Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3603784..3604325 Replicon chromosome
Accession NZ_CP035312
Organism Escherichia coli strain D72

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag ERL60_RS17585 Protein ID WP_000615976.1
Coordinates 3604047..3604325 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag ERL60_RS17580 Protein ID WP_000729704.1
Coordinates 3603784..3604044 (+) Length 87 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ERL60_RS17560 3599219..3599851 + 633 Protein_3387 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
ERL60_RS17565 3599835..3601928 + 2094 WP_021570012.1 flagellar biosynthesis protein FlhA -
ERL60_RS17570 3602152..3602649 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ERL60_RS17575 3602825..3603598 - 774 WP_015675129.1 C40 family peptidase -
ERL60_RS17580 3603784..3604044 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ERL60_RS17585 3604047..3604325 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ERL60_RS17590 3604481..3605221 + 741 WP_001225679.1 murein L,D-transpeptidase -
ERL60_RS17595 3605192..3605959 - 768 WP_000333379.1 class II glutamine amidotransferase -
ERL60_RS17600 3606165..3606743 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-91)

Antitoxin

(4-84)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T117598 WP_000615976.1 NZ_CP035312:3604047-3604325 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T117598 NZ_CP035312:3604047-3604325 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT117598 WP_000729704.1 NZ_CP035312:3603784-3604044 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT117598 NZ_CP035312:3603784-3604044 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB U9XPG0


Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References