Detailed information of TA system TA117597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3589339..3590033
• Replicon: chromosome
• Accession: NZ_CP035312
• Organism: Escherichia coli strain D72

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: ERL60_RS17490
• Protein ID: WP_001263493.1
• Coordinates: 3589339..3589737 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ERL60_RS17495
• Protein ID: WP_000554757.1
• Coordinates: 3589740..3590033 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERL60_RS17460	3584339..3585583	-	1245	WP_000189541.1	esterase FrsA	-
-	3584999..3585079	-	81	NuclAT_8	-	-
-	3584999..3585079	-	81	NuclAT_8	-	-
-	3584999..3585079	-	81	NuclAT_8	-	-
-	3584999..3585079	-	81	NuclAT_8	-	-
ERL60_RS17465	3585675..3586133	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ERL60_RS17470	3586394..3587851	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
ERL60_RS17475	3587908..3588429	-	522	Protein_3371	peptide chain release factor H	-
ERL60_RS17480	3588428..3588631	-	204	Protein_3372	RNA ligase RtcB family protein	-
ERL60_RS17485	3588877..3589329	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
ERL60_RS17490	3589339..3589737	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ERL60_RS17495	3589740..3590033	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ERL60_RS17500	3590085..3591140	-	1056	WP_001226164.1	DNA polymerase IV	-
ERL60_RS17505	3591211..3592134	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
ERL60_RS17510	3592137..3593000	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
ERL60_RS17515	3593013..3593729	-	717	WP_131419479.1	FliA/WhiG family RNA polymerase sigma factor	-
ERL60_RS17520	3593749..3594216	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T117597 WP_001263493.1 NZ_CP035312:c3589737-3589339 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T117597 NZ_CP035312:c3589737-3589339 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT117597 WP_000554757.1 NZ_CP035312:c3590033-3589740 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT117597 NZ_CP035312:c3590033-3589740 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6