Detailed information of TA system TA117567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 358756..359355
• Replicon: chromosome
• Accession: NZ_CP035300
• Organism: Dyella sp. M7H15-1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: EO087_RS01930
• Protein ID: WP_128897400.1
• Coordinates: 359074..359355 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EO087_RS01925
• Protein ID: WP_205744464.1
• Coordinates: 358756..359040 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO087_RS01900	353903..355108	+	1206	WP_128897394.1	phage tail sheath subtilisin-like domain-containing protein	-
EO087_RS01905	355108..355617	+	510	WP_128897395.1	phage major tail tube protein	-
EO087_RS01910	355619..355921	+	303	WP_164931732.1	phage tail assembly protein	-
EO087_RS01915	356032..358257	+	2226	WP_128897397.1	phage tail protein	-
EO087_RS01920	358262..358744	+	483	WP_128897398.1	phage tail protein	-
EO087_RS01925	358756..359040	-	285	WP_205744464.1	HigA family addiction module antidote protein	Antitoxin
EO087_RS01930	359074..359355	-	282	WP_128897400.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EO087_RS01935	359418..359630	+	213	WP_128897401.1	tail protein X	-
EO087_RS01940	359621..360703	+	1083	WP_128897402.1	phage late control D family protein	-
EO087_RS01945	360909..361874	-	966	WP_128897403.1	hypothetical protein	-
EO087_RS01950	362416..363339	+	924	WP_128897404.1	arginase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	322790..360703	37913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-61)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10520.12 Da
• Isoelectric Point: 9.3021

>T117567 WP_128897400.1 NZ_CP035300:c359355-359074 [Dyella sp. M7H15-1]
MAIQSFACKHTQALFEGGHPRKFRAFEDAAIRKLSQLDAALTLDFLRSPPGNMLEALKGDRKGQHSIRINGQFRLCFVWI
QAGPTHVEIVDYH

Nucleotide

• Length: 282 bp

>T117567 NZ_CP035300:c359355-359074 [Dyella sp. M7H15-1]
ATGGCTATCCAATCCTTCGCCTGCAAACACACGCAAGCCCTGTTCGAAGGCGGCCACCCGCGCAAATTTCGGGCGTTCGA
AGACGCTGCTATCCGCAAGCTCAGCCAACTGGACGCCGCCTTGACACTGGACTTCCTGCGCTCCCCGCCCGGCAACATGC
TCGAAGCCCTCAAAGGTGATCGCAAAGGCCAGCACAGCATCCGCATCAACGGCCAATTTCGGCTGTGCTTCGTGTGGATC
CAGGCAGGCCCTACCCACGTTGAAATTGTCGACTACCACTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10317.83 Da
• Isoelectric Point: 7.3187

>AT117567 WP_205744464.1 NZ_CP035300:c359040-358756 [Dyella sp. M7H15-1]
MRPVHPGEILREDFLAPISMSVNALSLALGVPATRIHEIVNERRAVTADTAERLARYFGGDAASWLALQAAYDLKTLPTH
DDILRRVIPRASNG

Nucleotide

• Length: 285 bp

>AT117567 NZ_CP035300:c359040-358756 [Dyella sp. M7H15-1]
ATGCGCCCCGTGCACCCCGGCGAGATCCTTCGGGAAGATTTCCTGGCCCCCATCAGCATGAGCGTCAACGCCCTTTCTCT
GGCGCTTGGTGTGCCCGCCACCCGCATACATGAAATCGTCAACGAACGCCGCGCCGTCACCGCCGACACAGCCGAGCGGC
TCGCCCGTTACTTCGGCGGCGATGCCGCAAGCTGGCTGGCGCTGCAAGCCGCCTACGATCTCAAAACACTGCCCACCCAC
GACGACATCCTGCGCCGTGTGATTCCGCGCGCATCCAACGGCTAG