Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2598451..2599013 Replicon chromosome
Accession NZ_CP035276
Organism Citrobacter freundii strain R17

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5QCT4
Locus tag EQ249_RS12915 Protein ID WP_003836234.1
Coordinates 2598735..2599013 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B5QC60
Locus tag EQ249_RS12910 Protein ID WP_003836231.1
Coordinates 2598451..2598735 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQ249_RS12895 2596142..2597026 + 885 WP_003833024.1 formate dehydrogenase N subunit beta -
EQ249_RS12900 2597019..2597675 + 657 WP_003020054.1 formate dehydrogenase-N subunit gamma -
EQ249_RS12905 2597805..2598407 + 603 WP_032935729.1 inorganic diphosphatase -
EQ249_RS12910 2598451..2598735 - 285 WP_003836231.1 HigA family addiction module antidote protein Antitoxin
EQ249_RS12915 2598735..2599013 - 279 WP_003836234.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EQ249_RS12920 2599234..2600949 - 1716 WP_106107369.1 ATP-binding cassette domain-containing protein -
EQ249_RS12925 2601258..2602955 - 1698 WP_003836239.1 malate dehydrogenase -
EQ249_RS12930 2603135..2603275 - 141 WP_003020039.1 stationary-phase-induced ribosome-associated protein -
EQ249_RS12935 2603503..2603934 + 432 WP_106107368.1 OsmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-83)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10637.28 Da        Isoelectric Point: 9.8965

>T117529 WP_003836234.1 NZ_CP035276:c2599013-2598735 [Citrobacter freundii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGDRDGIWAIAVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T117529 NZ_CP035276:c2599013-2598735 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAGGGATTGCGAGATTTGTTTCTTCATGGAAGAACATCAGGCGTTATGGCGAAACATGT
CCGACGATTACGACACAGGCTTGCCGTTATTGATGCGGCAAGCAAGGTTACAGATATCAATATGCCCGGTTATAAGCTAC
ATCCTCTGGTAGGTGACCGTGATGGCATATGGGCAATAGCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGACTACGAGGACTATCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10358.10 Da        Isoelectric Point: 8.8094

>AT117529 WP_003836231.1 NZ_CP035276:c2598735-2598451 [Citrobacter freundii]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQTTWSLAEAQ
KSVDISRLRKLAVQ

Download         Length: 285 bp

>AT117529 NZ_CP035276:c2598735-2598451 [Citrobacter freundii]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGAAGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAGATTGCACCTTCAACGGCAAGTCGACTATTAACCGGGAAAGCAGCCCTAACGCCTGAGATGGCGA
TTAAACTCTCTGTGGTGATTGGCAGCTCACCCGAAATGTGGTTAAACCTGCAAACTACCTGGAGTCTGGCAGAGGCCCAA
AAAAGCGTGGATATTTCACGCCTGCGTAAGCTGGCAGTACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5QCT4

Antitoxin

Source ID Structure
AlphaFold DB A0A8B5QC60

References