Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 1520026..1520538 Replicon chromosome
Accession NZ_CP035260
Organism Streptococcus pneumoniae strain TVO_1901925

Toxin (Protein)


Gene name yoeB Uniprot ID Q8DNR3
Locus tag EQH20_RS07535 Protein ID WP_000924721.1
Coordinates 1520026..1520280 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B7LSG7
Locus tag EQH20_RS07540 Protein ID WP_000388571.1
Coordinates 1520284..1520538 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH20_RS07520 (EQH20_07955) 1517127..1517441 - 315 WP_000979235.1 DNA-directed RNA polymerase subunit omega -
EQH20_RS07525 (EQH20_07960) 1517466..1518092 - 627 WP_000775047.1 guanylate kinase -
EQH20_RS07530 (EQH20_07965) 1518222..1519835 - 1614 WP_000404940.1 ribonuclease Y -
EQH20_RS07535 (EQH20_07970) 1520026..1520280 - 255 WP_000924721.1 Txe/YoeB family addiction module toxin Toxin
EQH20_RS07540 (EQH20_07975) 1520284..1520538 - 255 WP_000388571.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EQH20_RS07545 (EQH20_07980) 1520776..1521873 - 1098 WP_061647425.1 nucleotidyltransferase -
EQH20_RS07550 (EQH20_07985) 1521883..1522629 - 747 WP_000605154.1 class I SAM-dependent methyltransferase -
EQH20_RS07555 (EQH20_07990) 1522732..1523085 - 354 WP_001003001.1 ribosome silencing factor -
EQH20_RS07560 (EQH20_07995) 1523098..1523598 - 501 WP_000844707.1 cysteine hydrolase family protein -
EQH20_RS07565 (EQH20_08000) 1523599..1524192 - 594 WP_000121654.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
EQH20_RS07570 (EQH20_08005) 1524192..1524821 - 630 WP_000151307.1 nicotinate-nucleotide adenylyltransferase -
EQH20_RS07575 (EQH20_08010) 1524873..1525184 - 312 WP_000060169.1 ribosome assembly RNA-binding protein YhbY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(1-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-75)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10115.55 Da        Isoelectric Point: 7.9716

>T117494 WP_000924721.1 NZ_CP035260:c1520280-1520026 [Streptococcus pneumoniae]
MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGKPEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSF
KDHY

Download         Length: 255 bp

>T117494 NZ_CP035260:c1520280-1520026 [Streptococcus pneumoniae]
ATGCTACTCAAGTTTACAGAAGATGCGTGGGCAGATTATTGCTACTGGCAAAACCAAGATAAAAAAACACTAAAAAGAAT
CAATAAATTAATCAAGGATATTCAACGCGATCCATTTACAGGAATAGGAAAACCAGAGCCACTCAAATATGATTACCAAG
GAGCCTGGTCACGGCGTATTGATGCAGAAAATCGCTTGATTTATATGATGGATGGAGATAGCGTGGCTTTCTTGTCCTTT
AAAGATCATTACTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9718.05 Da        Isoelectric Point: 4.8430

>AT117494 WP_000388571.1 NZ_CP035260:c1520538-1520284 [Streptococcus pneumoniae]
MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKSEWDSIQETLRIAQNKELSDKVLRGMAQVRAGSIQVH
VIEE

Download         Length: 255 bp

>AT117494 NZ_CP035260:c1520538-1520284 [Streptococcus pneumoniae]
ATGGAAGCAGTCCTTTACTCAACATTCCGAAATCATTTAAAGGACTACATGAAGAAGGTAAATGATGAATTTGAGCCTTT
GACGGTGGTCAATAAAAATCCAGATGAGGACATTGTAGTTCTTTCAAAGAGCGAATGGGACAGTATTCAGGAAACCCTGA
GAATCGCTCAAAATAAGGAGTTATCAGACAAGGTTTTGCGAGGAATGGCTCAAGTCCGTGCTGGAAGCATTCAGGTCCAT
GTGATTGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7LEN4

Antitoxin

Source ID Structure
AlphaFold DB A0A0B7LSG7

References