Detailed information of TA system TA117433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 1603177..1603689
• Replicon: chromosome
• Accession: NZ_CP035236
• Organism: Streptococcus pneumoniae strain TVO_1902282

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q8DNR3
• Locus tag: EQH44_RS07960
• Protein ID: WP_000924721.1
• Coordinates: 1603177..1603431 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: K0ZY22
• Locus tag: EQH44_RS07965
• Protein ID: WP_000388572.1
• Coordinates: 1603435..1603689 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH44_RS07940 (EQH44_08410)	1598504..1598818	-	315	WP_000979235.1	DNA-directed RNA polymerase subunit omega	-
EQH44_RS07945 (EQH44_08415)	1598843..1599469	-	627	WP_000775037.1	guanylate kinase	-
EQH44_RS07950 (EQH44_08420)	1599599..1601212	-	1614	WP_000404938.1	ribonuclease Y	-
EQH44_RS07955 (EQH44_08425)	1601407..1602804	+	1398	WP_001812180.1	ISNCY-like element IS1202 family transposase	-
EQH44_RS10745	1602877..1603002	+	126	WP_001258158.1	hypothetical protein	-
EQH44_RS07960 (EQH44_08430)	1603177..1603431	-	255	WP_000924721.1	Txe/YoeB family addiction module toxin	Toxin
EQH44_RS07965 (EQH44_08435)	1603435..1603689	-	255	WP_000388572.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EQH44_RS07970 (EQH44_08440)	1603927..1605024	-	1098	WP_000156334.1	nucleotidyltransferase	-
EQH44_RS07975 (EQH44_08445)	1605034..1605774	-	741	WP_000257066.1	class I SAM-dependent methyltransferase	-
EQH44_RS07980 (EQH44_08450)	1606094..1606447	-	354	WP_001003001.1	ribosome silencing factor	-
EQH44_RS07985 (EQH44_08455)	1606460..1606960	-	501	WP_000844719.1	cysteine hydrolase family protein	-
EQH44_RS07990 (EQH44_08460)	1606961..1607554	-	594	WP_000121673.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQH44_RS07995 (EQH44_08465)	1607554..1608183	-	630	WP_000151309.1	nicotinate-nucleotide adenylyltransferase	-
EQH44_RS08000 (EQH44_08470)	1608235..1608546	-	312	WP_000060169.1	ribosome assembly RNA-binding protein YhbY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1601407..1602804	1397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10115.55 Da
• Isoelectric Point: 7.9716

>T117433 WP_000924721.1 NZ_CP035236:c1603431-1603177 [Streptococcus pneumoniae]
MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGKPEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSF
KDHY

Nucleotide

• Length: 255 bp

>T117433 NZ_CP035236:c1603431-1603177 [Streptococcus pneumoniae]
ATGCTACTCAAGTTTACAGAAGATGCCTGGGCAGATTATTGCTACTGGCAAAACCAAGATAAAAAAACACTAAAAAGAAT
CAATAAATTAATCAAGGATATTCAACGCGATCCATTTACAGGAATAGGAAAACCAGAGCCACTCAAATATGATTACCAAG
GAGCCTGGTCACGGCGTATTGATGCAGAAAATCGCTTGATTTATATGATGGATGGAGATAGCGTGGCTTTCTTGTCCTTT
AAAGATCATTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9706.00 Da
• Isoelectric Point: 4.8430

>AT117433 WP_000388572.1 NZ_CP035236:c1603689-1603435 [Streptococcus pneumoniae]
MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKSEWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVH
VIEE

Nucleotide

• Length: 255 bp

>AT117433 NZ_CP035236:c1603689-1603435 [Streptococcus pneumoniae]
ATGGAAGCAGTCCTTTACTCAACATTCCGAAATCATTTAAAGGACTACATGAAGAAGGTAAATGATGAATTTGAGCCTTT
GACGGTGGTCAATAAAAATCCAGATGAGGACATTGTAGTTCTTTCAAAGAGCGAATGGGACAGTATTCAGGAAACCCTGA
GAATCGCCCAAAATAAGGAGTTATCAGACAAGGTTTTGCGAGGAATGGCTCAAGTCCGTGCTGGAAGCACTCAGGTCCAT
GTGATTGAGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0B7LEN4

Antitoxin

• AlphaFold DB: K0ZY22