Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 73150..73685 Replicon plasmid unnamed1
Accession NZ_CP035221
Organism Enterococcus faecium strain SRCM103470

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag EQV91_RS14245 Protein ID WP_002314366.1
Coordinates 73150..73422 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag EQV91_RS14250 Protein ID WP_002297422.1
Coordinates 73422..73685 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQV91_RS14215 68585..69757 - 1173 WP_047928096.1 S8 family serine peptidase -
EQV91_RS14220 69787..70515 - 729 WP_002295679.1 peptidase -
EQV91_RS14225 70775..71323 - 549 WP_002292681.1 hypothetical protein -
EQV91_RS14230 71324..72181 - 858 WP_002292680.1 AAA family ATPase -
EQV91_RS14235 72467..72754 - 288 WP_002292679.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
EQV91_RS14240 72744..73073 - 330 WP_002292678.1 AbrB family transcriptional regulator -
EQV91_RS14245 73150..73422 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
EQV91_RS14250 73422..73685 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EQV91_RS14255 73919..74827 + 909 Protein_70 IS982-like element ISEfm1 family transposase -
EQV91_RS14260 74830..76005 - 1176 WP_010728556.1 MFS transporter -
EQV91_RS14265 76008..78257 - 2250 WP_002342422.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL - 1..239262 239262
- flank IS/Tn - - 68077..68661 584


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T117403 WP_002314366.1 NZ_CP035221:c73422-73150 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T117403 NZ_CP035221:c73422-73150 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT117403 WP_002297422.1 NZ_CP035221:c73685-73422 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT117403 NZ_CP035221:c73685-73422 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References